Key features and details
- Mouse monoclonal [D/2/D6/B7] to Flavivirus NS1
- Suitable for: ICC/IF, WB
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
- Isotype: IgG1
Product nameAnti-Flavivirus NS1 antibody [D/2/D6/B7]
DescriptionMouse monoclonal [D/2/D6/B7] to Flavivirus NS1
SpecificityThis monoclonal antibody reacts with a conformational epitope present on DENV1, 2, 3, 4, ZIKV, JEV and WNV NS1.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
Other Immunogen Type corresponding to Dengue virus 2 Flavivirus NS1. FN1 was created by immunizing mice with culture supernatant containing DENV 2 strain 16681, which was then boosted with cell lysate containing DENV 2 16681.
Database link: P29991
- WB: DENV1 (Hawaii), DENV2 (NGC), DENV2 (16681), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4, ZIKV infected cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Proclin 150
Constituent: 10% Fetal Bovine Serum (FBS)
Concentration information loading...
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab214337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.
Detects DENV1, 2 and ZIKV in immunofluorescence (weak detection of DENV4). Detects DENV1, 2, 3 and 4 foci in FRNT. Not tested against ZIKV, JEV and WNV for foci staining.
|WB||Use at an assay dependent concentration.
Primarily used for the detection of dimeric DENV1, 2, 3, 4, JEV and WNV NS1 protein band by western blot (non-reducing unheated conditions).
All lanes : Anti-Flavivirus NS1 antibody [D/2/D6/B7] (ab214337)
Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
Lane 3 : DENV2 (16681) infected C6/36 cell lysate
Lane 4 : DENV3 (H89) infected C6/36 cell lysate
Lane 5 : DENV4 (H241) infected C6/36 cell lysate
Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
Lane 7 : WNV (E101) infected C6/36 cell lysate
Lane 8 : YFV (17D) infected C6/36 cell lysate
Lane 9 : uninfected C6/36 cell lysate control
Immunofluorescent analysis of DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells labeling Flavivirus NS1 protein using ab214337.
ab214337 has been referenced in 1 publication.
- Furnon W et al. Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes. Viruses 11:N/A (2019). PubMed: 31569658