Anti-Flavivirus NS1 antibody [D/2/D6/B7] (ab214337)

Overview

  • Product name

    Anti-Flavivirus NS1 antibody [D/2/D6/B7]
  • Description

    Mouse monoclonal [D/2/D6/B7] to Flavivirus NS1
  • Host species

    Mouse
  • Specificity

    This monoclonal antibody reacts with a conformational epitope present on DENV1, 2, 3, 4, ZIKV, JEV and WNV NS1.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
  • Immunogen

    Other Immunogen Type corresponding to Dengue Virus 1 Flavivirus NS1. DENV infected supernatant/ lysate.

  • Positive control

    • WB: DENV1 (Hawaii), DENV2 (NGC), DENV2 (16681), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4, ZIKV infected cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Proclin
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    D/2/D6/B7
  • Isotype

    IgG1
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab214337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Detects DENV1, 2 and ZIKV in immunofluorescence (weak detection of DENV4). Detects DENV1, 2, 3 and 4 foci in FRNT. Not tested against ZIKV, JEV and WNV for foci staining.

WB Use at an assay dependent concentration.

Primarily used for the detection of dimeric DENV1, 2, 3, 4, JEV and WNV NS1 protein band by western blot (non-reducing unheated conditions).

Images

  • All lanes : Anti-Flavivirus NS1 antibody [D/2/D6/B7] (ab214337)

    Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
    Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
    Lane 3 : DENV2 (16681) infected C6/36 cell lysate
    Lane 4 : DENV3 (H89) infected C6/36 cell lysate
    Lane 5 : DENV4 (H241) infected C6/36 cell lysate
    Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
    Lane 7 : WNV (E101) infected C6/36 cell lysate
    Lane 8 : YFV (17D) infected C6/36 cell lysate
    Lane 9 : uninfected C6/36 cell lysate control
  • Immunofluorescent analysis of DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells labeling Flavivirus NS1 protein using ab214337.

References

ab214337 has not yet been referenced specifically in any publications.

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