Anti-Flavivirus NS1 antibody [D/2/D6/B7] (ab214337)
Key features and details
- Mouse monoclonal [D/2/D6/B7] to Flavivirus NS1
- Suitable for: ICC/IF, WB
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
- Isotype: IgG1
Overview
-
Product name
Anti-Flavivirus NS1 antibody [D/2/D6/B7] -
Description
Mouse monoclonal [D/2/D6/B7] to Flavivirus NS1 -
Host species
Mouse -
Specificity
This monoclonal antibody reacts with a conformational epitope present on DENV1, 2, 3, 4, ZIKV, JEV and WNV NS1. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus -
Immunogen
Tissue, cells or virus corresponding to Dengue virus 2 Flavivirus NS1. FN1 was created by immunizing mice with culture supernatant containing DENV 2 strain 16681, which was then boosted with cell lysate containing DENV 2 16681.
Database link: P29991 -
Positive control
- WB: DENV1 (Hawaii), DENV2 (NGC), DENV2 (16681), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4, ZIKV infected cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Proclin 150
Constituents: 10% Fetal Bovine Serum (FBS), 89.9% RPMI 1640 -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
D/2/D6/B7 -
Isotype
IgG1 -
Light chain type
kappa
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
Detects DENV1, 2 and ZIKV in immunofluorescence (weak detection of DENV4). Detects DENV1, 2, 3 and 4 foci in FRNT. Not tested against ZIKV, JEV and WNV for foci staining. |
|
WB |
Use at an assay dependent concentration.
Primarily used for the detection of dimeric DENV1, 2, 3, 4, JEV and WNV NS1 protein band by western blot (non-reducing unheated conditions). |
Notes |
---|
ICC/IF
Use at an assay dependent concentration. Detects DENV1, 2 and ZIKV in immunofluorescence (weak detection of DENV4). Detects DENV1, 2, 3 and 4 foci in FRNT. Not tested against ZIKV, JEV and WNV for foci staining. |
WB
Use at an assay dependent concentration. Primarily used for the detection of dimeric DENV1, 2, 3, 4, JEV and WNV NS1 protein band by western blot (non-reducing unheated conditions). |
Images
-
All lanes : Anti-Flavivirus NS1 antibody [D/2/D6/B7] (ab214337)
Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
Lane 3 : DENV2 (16681) infected C6/36 cell lysate
Lane 4 : DENV3 (H89) infected C6/36 cell lysate
Lane 5 : DENV4 (H241) infected C6/36 cell lysate
Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
Lane 7 : WNV (E101) infected C6/36 cell lysate
Lane 8 : YFV (17D) infected C6/36 cell lysate
Lane 9 : uninfected C6/36 cell lysate control -
Immunofluorescent analysis of DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells labeling Flavivirus NS1 protein using ab214337.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab214337 has been referenced in 4 publications.
- Warner NL et al. Unbiased Identification of Dengue Virus Non-Structural Protein 1 Peptides for Use in Vaccine Design. Vaccines (Basel) 10:N/A (2022). PubMed: 36560438
- Westrich JA et al. Characterization of subclinical ZIKV infection in immune-competent guinea pigs and mice. J Gen Virol 102:N/A (2021). PubMed: 34410903
- Yamanaka A et al. Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012. mSphere 6:e0033921 (2021). PubMed: 34259560
- Furnon W et al. Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes. Viruses 11:N/A (2019). PubMed: 31569658