Overview

  • Product name

    Anti-FLCN antibody [EPNCIR147]
    See all FLCN primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR147] to FLCN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human FLCN. The exact sequence is proprietary.

  • Positive control

    • 293T and NCCIT cell lysates
  • General notes

    This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Marston Linehan. View antibodies from NCI Center for Cancer Research Collaboration.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR147
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      May play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). May be a tumor suppressor. May be involved in colorectal tumorigenesis. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
    • Tissue specificity

      Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.
    • Involvement in disease

      Defects in FLCN are the cause of Birt-Hogg-Dube syndrome (BHD) [MIM:135150]. BHD is a rare autosomal dominant genodermatosis characterized by hair follicle hamartomas (fibrofolliculomas), kidney tumors, and spontaneous pneumothorax. Fibrofolliculomas are part of the triad of BHD skin lesions that also includes trichodiscomas and acrochordons. Onset of this dermatologic condition is invariably in adulthood. BHD is associated with a variety of histologic types of renal tumors, including chromophobe renal cell carcinoma (RCC), benign renal oncocytoma, clear-cell RCC and papillary type I RCC. Multiple lipomas, angiolipomas, and parathyroid adenomas are also seen in patients affected with this disease. The majority of mutations are predicted to prematurely terminate the protein.
      Defects in FLCN are in some cases a cause of primary spontaneous pneumothorax (PSP) [MIM:173600]. PSP is a condition in which air is present in the pleural space in the absence of a precipitating event, such as trauma or lung disease. This results in secondary collapse of the lung, either partially or completely, and some degree of hypoxia. PSP is relatively common, with an incidence between 7.4-18/100'000 for men and 1.2-6/100'000 for women and a dose-dependent, increased risk among smokers. Most cases are sporadic, typically occurring in tall, thin men aged 10-30 years and generally while at rest. Familial PSP is rarer and usually is inherited as an autosomal dominant condition with reduced penetrance, although X-linked recessive and autosomal recessive inheritance have also been suggested.
      Note=Defects in FLCN may be involved in renal cell carcinoma.
    • Sequence similarities

      Belongs to the folliculin family.
    • Developmental stage

      Expressed in fetal lung, kidney, liver, and brain.
    • Post-translational
      modifications

      Phosphorylated. Several different phosphorylated forms exist.
    • Cellular localization

      Cytoplasm. Nucleus. Mainly localized in the nucleus. Co-localizes with FNIP1 and FNIP2 in the cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • BHD antibody
      • BHD skin lesion fibrofolliculoma protein antibody
      • Birt Hogg Dube syndrome protein antibody
      • Birt-Hogg-Dube syndrome protein antibody
      • DKFZp547A118 antibody
      • FLCL antibody
      • Flcn antibody
      • FLCN_HUMAN antibody
      • FLJ45004 antibody
      • FLJ99377 antibody
      • Folliculin antibody
      • MGC17998 antibody
      • MGC23445 antibody
      see all

    Images

    • All lanes : Anti-FLCN antibody [EPNCIR147] (ab124885) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 64 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Li J  et al. Myeloid Folliculin balances mTOR activation to maintain innate immunity homeostasis. JCI Insight 5:N/A (2019). Read more (PubMed: 30843872) »
    • Wada S  et al. The tumor suppressor FLCN mediates an alternate mTOR pathway to regulate browning of adipose tissue. Genes Dev 30:2551-2564 (2016). Read more (PubMed: 27913603) »
    See all 2 Publications for this product

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