Anti-FLI1 antibody - C-terminal (ab180902)
Key features and details
- Rabbit polyclonal to FLI1 - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-FLI1 antibody - C-terminal
See all FLI1 primary antibodies -
Description
Rabbit polyclonal to FLI1 - C-terminal -
Host species
Rabbit -
Specificity
At least four isoforms of FLI1 are known to exist; this antibody will detect all four. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FLI1 (C terminal). The exact sequence is proprietary. 17 amino acid peptide near the C-terminus of Human FLI1.
Database link: Q01543 -
Positive control
- Jurkat whole cell lysate (ab7899); HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. -
Involvement in disease
Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2313 Human
- Omim: 193067 Human
- SwissProt: Q01543 Human
- Unigene: 504281 Human
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Alternative names
- ERGB transcription factor antibody
- Ewing Sarcoma breakpoint region 2 antibody
- EWSR 2 antibody
see all
Images
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Lane 1 : Anti-FLI1 antibody - C-terminal (ab180902) at 0.5 µg/ml
Lane 2 : Anti-FLI1 antibody - C-terminal (ab180902) at 1 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 51 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescent analysis of HeLa cells labeling FLI1 with ab180902 at 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab180902 has been referenced in 1 publication.
- Su S et al. SPOP and OTUD7A Control EWS-FLI1 Protein Stability to Govern Ewing Sarcoma Growth. Adv Sci (Weinh) 8:e2004846 (2021). PubMed: 34060252