Key features and details
- Rabbit polyclonal to FLI1 - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-FLI1 antibody - C-terminal
See all FLI1 primary antibodies
DescriptionRabbit polyclonal to FLI1 - C-terminal
SpecificityAt least four isoforms of FLI1 are known to exist; this antibody will detect all four.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human FLI1 (C terminal). The exact sequence is proprietary. 17 amino acid peptide near the C-terminus of Human FLI1.
Database link: Q01543
- Jurkat whole cell lysate (ab7899); HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).|
FunctionSequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Involvement in diseaseDefects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
- Information by UniProt
- ERGB transcription factor antibody
- Ewing Sarcoma breakpoint region 2 antibody
- EWSR 2 antibody
Lane 1 : Anti-FLI1 antibody - C-terminal (ab180902) at 0.5 µg/ml
Lane 2 : Anti-FLI1 antibody - C-terminal (ab180902) at 1 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 51 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescent analysis of HeLa cells labeling FLI1 with ab180902 at 5 µg/ml.
ab180902 has not yet been referenced specifically in any publications.