Product nameAnti-FLI1 antibody - ChIP Grade
See all FLI1 primary antibodies
DescriptionRabbit polyclonal to FLI1 - ChIP Grade
Tested applicationsSuitable for: ChIP, WB, IP, CHIPseq, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human FLI1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q01543
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab15289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 19289502|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|IP||Use a concentration of 2 µg/ml.|
|CHIPseq||Use at an assay dependent concentration. PubMed: 24760698|
|IHC-P||1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
FunctionSequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Involvement in diseaseDefects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
- Information by UniProt
- ERGB transcription factor antibody
- Ewing Sarcoma breakpoint region 2 antibody
- EWSR 2 antibody
Chromatin was prepared from nuclear lysate of the mouse haematopoietic progenitor cell line HPC-7. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 10 minutes in 0.4% and 1% formaldehyde. The primary antibody was used undiluted and incubated with the sample for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR.
All lanes : Anti-FLI1 antibody - ChIP Grade (ab15289) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Additional bands at: 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab15289, at 1/50 dilution, staining FLI1 in angiosarcoma by Immunohistochemistry (FFPE-sections).
This product has been referenced in:
- Machiela MJ et al. Genome-wide association study identifies multiple new loci associated with Ewing sarcoma susceptibility. Nat Commun 9:3184 (2018). Read more (PubMed: 30093639) »
- Johnson KM et al. Role for the EWS domain of EWS/FLI in binding GGAA-microsatellites required for Ewing sarcoma anchorage independent growth. Proc Natl Acad Sci U S A 114:9870-9875 (2017). Read more (PubMed: 28847958) »