RecombinantRabMAb

Recombinant Anti-FLI1 antibody [EPR4645] (ab124791)

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Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
  • Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
  • Anti-FLI1 antibody [EPR4645] (ab124791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4645] to FLI1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-FLI1 antibody [EPR4645]
    See all FLI1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4645] to FLI1

  • Host species

    Rabbit

  • Specificity

    This product does not cross-react with ERG.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment

  • Immunogen

    Synthetic peptide within Human FLI1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Raw264.7, U937, Jurkat, K562 and C6 cell lysates. FLI1 recombinant protein.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

    • Involvement in disease

      Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.

    • Sequence similarities

      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ERGB transcription factor antibody
      • Ewing Sarcoma breakpoint region 2 antibody
      • EWSR 2 antibody
      • EWSR2 antibody
      • FLI 1 antibody
      • FLI 1 proto oncogene antibody
      • Fli-1 proto-oncogene, ETS transcription factor antibody
      • FLI1 antibody
      • FLI1 EWS fusion gene antibody
      • FLI1 proto oncogene antibody
      • FLI1_HUMAN antibody
      • Friend leukemia integration 1 (FLI1) transcription factor antibody
      • Friend leukemia integration 1 transcription factor antibody
      • Friend leukemia virus integration 1 antibody
      • Proto-oncogene Fli-1 antibody
      • Sarcoma breakpoint region 2 (EWSR2) antibody
      • SIC 1 antibody
      • SIC1 antibody
      • Transcription factor ERGB antibody
      • Viral integration region FLI1 antibody
      • Viral integration region FLI1, mouse, homolog of antibody
      see all

    Images

    • Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
      Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
      All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution

      Lane 1 : Raw264.7 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 51 kDa

    • Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
      Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
      All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution

      Lane 1 : ERG recombinant protein
      Lane 2 : FLI1 recombinant protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 51 kDa

    • Anti-FLI1 antibody [EPR4645] (ab124791)
      Anti-FLI1 antibody [EPR4645] (ab124791)

    References (0)

    Publishing research using ab124791? Please let us know so that we can cite the reference in this datasheet.

    ab124791 has not yet been referenced specifically in any publications.

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