Recombinant Anti-FLI1 antibody [EPR4645] (ab124791)
![Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)](https://www.abcam.com/ps/products/124/ab124791/Images/ab124791-138676-anti-fli1-antibody-epr4645-western-blot.jpg "Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4645] to FLI1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-FLI1 antibody [EPR4645]
See all FLI1 primary antibodies -
Description
Rabbit monoclonal [EPR4645] to FLI1 -
Host species
Rabbit -
Specificity
This product does not cross-react with ERG. -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human FLI1 aa 50-150. The exact sequence is proprietary.
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Positive control
- Raw264.7, U937, Jurkat, K562 and C6 cell lysates. FLI1 recombinant protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4645 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 51 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
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Function
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. -
Involvement in disease
Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2313 Human
- Entrez Gene: 14247 Mouse
- Entrez Gene: 315532 Rat
- Omim: 193067 Human
- SwissProt: Q01543 Human
- SwissProt: P26323 Mouse
- Unigene: 504281 Human
- Unigene: 258908 Mouse
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Alternative names
- ERGB transcription factor antibody
- Ewing Sarcoma breakpoint region 2 antibody
- EWSR 2 antibody
see all
Images
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Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution
Lane 1 : Raw264.7 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lane 5 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa -
![Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)](https://www.abcam.com/ps/products/124/ab124791/Images/ab124791-138675-anti-fli1-antibody-epr4645-western-blot.jpg)
Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution
Lane 1 : ERG recombinant protein
Lane 2 : FLI1 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa -
![Anti-FLI1 antibody [EPR4645] (ab124791)](https://www.abcam.com/ps/products/124/ab124791/Images/ab124791-3-benefits-of-recombinant-antibodies.png)
Anti-FLI1 antibody [EPR4645] (ab124791)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124791 has been referenced in 1 publication.
- Ross NT et al. CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma. Nat Chem Biol 16:50-59 (2020). PubMed: 31819276