- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-FLI1 antibody [EPR4645]
See all FLI1 primary antibodies -
DescriptionRabbit monoclonal [EPR4645] to FLI1
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Host speciesRabbit
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SpecificityThis product does not cross-react with ERG.
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human FLI1 aa 50-150. The exact sequence is proprietary.
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Positive control
- Raw264.7, U937, Jurkat, K562 and C6 cell lysates. FLI1 recombinant protein.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4645
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 51 kDa. |
Target
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FunctionSequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
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Involvement in diseaseDefects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
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Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 2313 Human
- Entrez Gene: 14247 Mouse
- Entrez Gene: 315532 Rat
- Omim: 193067 Human
- SwissProt: Q01543 Human
- SwissProt: P26323 Mouse
- Unigene: 504281 Human
- Unigene: 258908 Mouse
see all -
Alternative names
- ERGB transcription factor antibody
- Ewing Sarcoma breakpoint region 2 antibody
- EWSR 2 antibody
see all
Images
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![Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)](https://www.abcam.com/ps/datasheet/images/124/ab124791/FLI1-Primary-antibodies-ab124791-1.JPG)
Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution
Lane 1 : Raw264.7 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lane 5 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa -
![Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)](https://www.abcam.com/ps/datasheet/images/124/ab124791/FLI1-Primary-antibodies-ab124791-2.JPG)
Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution
Lane 1 : ERG recombinant protein
Lane 2 : FLI1 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa
Protocols
References
ab124791 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"