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Cell Biology Apoptosis Nucleus Other
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RecombinantRabMAb

Recombinant Anti-FLI1 antibody [EPR4645] (ab124791)

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Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
  • Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
  • Anti-FLI1 antibody [EPR4645] (ab124791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4645] to FLI1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

You may also be interested in

Protein
Product image
Recombinant Human FLI1 protein (ab132086)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-FLI1 antibody [EPR4645]
    See all FLI1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4645] to FLI1
  • Host species

    Rabbit
  • Specificity

    This product does not cross-react with ERG.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Immunogen

    Synthetic peptide within Human FLI1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Raw264.7, U937, Jurkat, K562 and C6 cell lysates. FLI1 recombinant protein.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4645
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Ets
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-FLI1 antibody [EPR4645] - BSA and Azide free (ab248006)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 nuclear extract lysate (ab14851)
    • U937 nuclear extract lysate (TPA stimulated) (ab14906)
  • Recombinant Protein

    • Recombinant Human FLI1 protein (ab132086)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
    • Involvement in disease

      Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
    • Sequence similarities

      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q01543 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2313 Human
      • Entrez Gene: 14247 Mouse
      • Entrez Gene: 315532 Rat
      • Omim: 193067 Human
      • SwissProt: Q01543 Human
      • SwissProt: P26323 Mouse
      • Unigene: 504281 Human
      • Unigene: 258908 Mouse
      • Unigene: 203677 Rat
      see all
    • Alternative names

      • ERGB transcription factor antibody
      • Ewing Sarcoma breakpoint region 2 antibody
      • EWSR 2 antibody
      • EWSR2 antibody
      • FLI 1 antibody
      • FLI 1 proto oncogene antibody
      • Fli-1 proto-oncogene, ETS transcription factor antibody
      • FLI1 antibody
      • FLI1 EWS fusion gene antibody
      • FLI1 proto oncogene antibody
      • FLI1_HUMAN antibody
      • Friend leukemia integration 1 (FLI1) transcription factor antibody
      • Friend leukemia integration 1 transcription factor antibody
      • Friend leukemia virus integration 1 antibody
      • Proto-oncogene Fli-1 antibody
      • Sarcoma breakpoint region 2 (EWSR2) antibody
      • SIC 1 antibody
      • SIC1 antibody
      • Transcription factor ERGB antibody
      • Viral integration region FLI1 antibody
      • Viral integration region FLI1, mouse, homolog of antibody
      see all

    Images

    • Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
      Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
      All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution

      Lane 1 : Raw264.7 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 51 kDa

    • Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
      Western blot - Anti-FLI1 antibody [EPR4645] (ab124791)
      All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution

      Lane 1 : ERG recombinant protein
      Lane 2 : FLI1 recombinant protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 51 kDa

    • Anti-FLI1 antibody [EPR4645] (ab124791)
      Anti-FLI1 antibody [EPR4645] (ab124791)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab124791? Please let us know so that we can cite the reference in this datasheet.

    ab124791 has not yet been referenced specifically in any publications.

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