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RecombinantRabMAb

Recombinant Anti-FLI1 antibody [EPR4646] (ab133485)

Reviews (1)Q&A (2)References (16)
Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)
  • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
  • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
  • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)
  • Anti-FLI1 antibody [EPR4646] (ab133485)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4646] to FLI1
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-FLI1 antibody [EPR4646]
    See all FLI1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4646] to FLI1

  • Host species

    Rabbit

  • Specificity

    ab133485 does not cross-react with ERG.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment

  • Immunogen

    Synthetic peptide within Human FLI1 aa 350-450. The exact sequence is proprietary.
    (Peptide available as ab187137)

  • Positive control

    • U937, Jurkat, Raw264.7 and HL-60 cell lysates; Human Ewing sarcoma tissue; FLI1 recombinant protein

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/700. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/140.
WB
1/1000. Predicted molecular weight: 51 kDa.

For unpurified, use 1/200.
Notes
IHC-P
1/700. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/140.
WB
1/1000. Predicted molecular weight: 51 kDa.

For unpurified, use 1/200.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

  • Involvement in disease

    Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.

  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • ERGB transcription factor antibody
      • Ewing Sarcoma breakpoint region 2 antibody
      • EWSR 2 antibody
      • EWSR2 antibody
      • FLI 1 antibody
      • FLI 1 proto oncogene antibody
      • Fli-1 proto-oncogene, ETS transcription factor antibody
      • FLI1 antibody
      • FLI1 EWS fusion gene antibody
      • FLI1 proto oncogene antibody
      • FLI1_HUMAN antibody
      • Friend leukemia integration 1 (FLI1) transcription factor antibody
      • Friend leukemia integration 1 transcription factor antibody
      • Friend leukemia virus integration 1 antibody
      • Proto-oncogene Fli-1 antibody
      • Sarcoma breakpoint region 2 (EWSR2) antibody
      • SIC 1 antibody
      • SIC1 antibody
      • Transcription factor ERGB antibody
      • Viral integration region FLI1 antibody
      • Viral integration region FLI1, mouse, homolog of antibody
      see all

    Images

    • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
      Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
      Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (purified) + Jurkat cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit at 1/1000 dilution

      Predicted band size: 51 kDa
      Observed band size: 51 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

      Immunohistochemical staining of paraffin embedded human lung with purified ab133485 at a working dilution of 1 in 700. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
      Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
      Anti-FLI1 antibody [EPR4646] (ab133485) at 1/200 dilution (unpurified) + Jurkat cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 51 kDa
      Observed band size: 51 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
      Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
      All lanes : Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (unpurified)

      Lane 1 : U937 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Raw264.7 cell lysate
      Lane 4 : HL-60 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 51 kDa

    • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
      Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
      All lanes : Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (unpurified)

      Lane 1 : FLI1 recombinant protein
      Lane 2 : ERG
      recombinant protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 51 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

      Immunohistochemical staining of paraffin embedded human lung with unpurified ab133485 at a working dilution of 1 in 140. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

      Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labelled with unpurified ab133485 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-FLI1 antibody [EPR4646] (ab133485)
      Anti-FLI1 antibody [EPR4646] (ab133485)

    References (16)

    Publishing research using ab133485? Please let us know so that we can cite the reference in this datasheet.

    ab133485 has been referenced in 16 publications.

    • Wang C  et al. FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome. Front Immunol 12:607836 (2021). PubMed: 33717090
    • Aynaud MM  et al. Transcriptional Programs Define Intratumoral Heterogeneity of Ewing Sarcoma at Single-Cell Resolution. Cell Rep 30:1767-1779.e6 (2020). PubMed: 32049009
    • Flores G  et al. CDK9 Blockade Exploits Context-dependent Transcriptional Changes to Improve Activity and Limit Toxicity of Mithramycin for Ewing Sarcoma. Mol Cancer Ther 19:1183-1196 (2020). PubMed: 32127464
    • Fang Y  et al. Tbx20 Induction Promotes Zebrafish Heart Regeneration by Inducing Cardiomyocyte Dedifferentiation and Endocardial Expansion. Front Cell Dev Biol 8:738 (2020). PubMed: 32850848
    • Wang C  et al. FLI1 promotes protein translation via the transcriptional regulation of MKNK1 expression. Int J Oncol 56:430-438 (2020). PubMed: 31894299
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-FLI1 antibody [EPR4646]

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Ewing Sarcoma Cell Lines)
    Total protein in input
    200 µg
    Immuno-precipitation step
    Protein G
    Specification
    Ewing Sarcoma Cell Lines
    Read More

    DR. Utkarsh Kapoor

    Verified customer

    Submitted Sep 30 2021

    Question

    Dear Technical Team,

    Hope this e-mail finds you well!

    Kindly suggest the volume per ml along with no. of tests to be performed with our below abcam products as desired by the customer. The customer wants to perform IHC on deparaffinized sections :







    Cat No.



    Description



    Pack Size





    ab2074



    Rabbit polyclonal to CXCR4



    100 µg





    ab49124



    Rabbit polyclonal to SDF1



    50 µg





    ab108297



    Rabbit monoclonal [EPR3096] to CD99



    100 µl





    ab133485



    Rabbit monoclonal [EPR4646] to FLI1



    100 µl





    ab16667



    Rabbit monoclonal [SP6] to Ki67 - Proliferation Marker



    500 µl











    Looking forward towards your valued response on it.

    With Best Regards,

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.

    Each antibody should be titrated to determine the optimal working dilutions. It is the customer's responsibility to calculate how much product is needed for each assay.

    The required quantity of the antibody depends on many factors:

    1. dilution factor of the antibody,

    2. expression levels of the target,

    3. how you carry out the assay (in duplicates, triplicates or in quadruplicates),

    4. presence of positive and negative control,

    5. surface area of the tissue section on the slides etc.





    https://www.abcam.com/ab2074 - unit size: 100 µg at 1mg/ml; recommended starting dilution 1/50 or 1/100,

    https://www.abcam.com/ab49124 - only tested in Western blot application,

    https://www.abcam.com/ab108297 - Tissue culture supernatant (conc not provided); recommended starting dilution 1/250 - 1/500,

    https://www.abcam.com/ab133485 - Tissue culture supernatant (conc not provided); recommended starting dilution 1/250 - 1/500,

    https://www.abcam.com/ab16667 - Tissue culture supernatant (conc not provided); recommended starting dilution1/100



    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    Hope this e-mail finds you well!
    Kindly advise how many tests can be performed with our below abcam products as desired by the customer :
    Cat No.
    Description
    Pack Size
    ab10403
    Mouse monoclonal [44716.111] to CXCR4
    100 µg
    ab133485
    Rabbit monoclonal [EPR4646] to FLI1
    100 µl
    ab9087
    Mouse monoclonal [MEM-131] to CD99
    100 µg
    ab1101
    Mouse monoclonal to p53
    50 µg
    Looking forward towards your valued response on it.

    With Best Regards,

    Read More
    Answer

    Thank you for contacting us.

    The number of tests totally depends on the dilution or concentration range customer uses. However at the recommended dilution, the antibody can be used for 100 ICC/IF or 100 IHC-P or 100 WB tests.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

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