Recombinant
RabMAb

Recombinant Anti-FLI1 antibody [EPR4646] (ab133485)

Submit a review Q&A (2)References (7)

Overview

  • Product name

    Anti-FLI1 antibody [EPR4646]
    See all FLI1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4646] to FLI1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human FLI1 aa 350-450. The exact sequence is proprietary.
    (Peptide available as ab187137)

  • Positive control

    • U937, Jurkat, Raw264.7 and HL-60 cell lysates; Human Ewing sarcoma tissue; FLI1 recombinant protein

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab133485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/700. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/140.
WB 1/1000. Predicted molecular weight: 51 kDa.

For unpurified, use 1/200.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

    • Involvement in disease

      Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.

    • Sequence similarities

      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • ERGB transcription factor antibody
        • Ewing Sarcoma breakpoint region 2 antibody
        • EWSR 2 antibody
        • EWSR2 antibody
        • FLI 1 antibody
        • FLI 1 proto oncogene antibody
        • Fli-1 proto-oncogene, ETS transcription factor antibody
        • FLI1 antibody
        • FLI1 EWS fusion gene antibody
        • FLI1 proto oncogene antibody
        • FLI1_HUMAN antibody
        • Friend leukemia integration 1 (FLI1) transcription factor antibody
        • Friend leukemia integration 1 transcription factor antibody
        • Friend leukemia virus integration 1 antibody
        • Proto-oncogene Fli-1 antibody
        • Sarcoma breakpoint region 2 (EWSR2) antibody
        • SIC 1 antibody
        • SIC1 antibody
        • Transcription factor ERGB antibody
        • Viral integration region FLI1 antibody
        • Viral integration region FLI1, mouse, homolog of antibody
        see all

      Images

      • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
        Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
        Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (purified) + Jurkat cell lysate at 10 µg

        Secondary
        HRP goat anti-rabbit at 1/1000 dilution

        Predicted band size: 51 kDa
        Observed band size: 51 kDa



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

        Immunohistochemical staining of paraffin embedded human lung with purified ab133485 at a working dilution of 1 in 700. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

      • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
        Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
        Anti-FLI1 antibody [EPR4646] (ab133485) at 1/200 dilution (unpurified) + Jurkat cell lysate at 10 µg

        Secondary
        HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 51 kDa
        Observed band size: 51 kDa



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
        Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
        All lanes : Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (unpurified)

        Lane 1 : U937 cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : Raw264.7 cell lysate
        Lane 4 : HL-60 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 51 kDa

      • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
        Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)
        All lanes : Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (unpurified)

        Lane 1 : FLI1 recombinant protein
        Lane 2 : ERG
        recombinant protein

        Lysates/proteins at 0.01 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 51 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

        Immunohistochemical staining of paraffin embedded human lung with unpurified ab133485 at a working dilution of 1 in 140. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

        Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labelled with unpurified ab133485 at 1/250 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Wang N  et al. Selective ERK1/2 agonists isolated from Melia azedarach with potent anti-leukemic activity. BMC Cancer 19:764 (2019). Read more (PubMed: 31375085) »
      • Bergiers I  et al. Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis. Elife 7:N/A (2018). Read more (PubMed: 29555020) »
      See all 7 Publications for this product

      Publishing research using ab133485? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Dear Technical Team,

      Hope this e-mail finds you well!

      Kindly suggest the volume per ml along with no. of tests to be performed with our below abcam products as desired by the customer. The customer wants to perform IHC on deparaffinized sections :







      Cat No.



      Description



      Pack Size





      ab2074



      Rabbit polyclonal to CXCR4



      100 µg





      ab49124



      Rabbit polyclonal to SDF1



      50 µg





      ab108297



      Rabbit monoclonal [EPR3096] to CD99



      100 µl





      ab133485



      Rabbit monoclonal [EPR4646] to FLI1



      100 µl





      ab16667



      Rabbit monoclonal [SP6] to Ki67 - Proliferation Marker



      500 µl











      Looking forward towards your valued response on it.

      With Best Regards,

      Read More
      Answer

      Thank you for your enquiry and your interest in our products.

      Each antibody should be titrated to determine the optimal working dilutions. It is the customer's responsibility to calculate how much product is needed for each assay.

      The required quantity of the antibody depends on many factors:

      1. dilution factor of the antibody,

      2. expression levels of the target,

      3. how you carry out the assay (in duplicates, triplicates or in quadruplicates),

      4. presence of positive and negative control,

      5. surface area of the tissue section on the slides etc.





      https://www.abcam.com/ab2074 - unit size: 100 µg at 1mg/ml; recommended starting dilution 1/50 or 1/100,

      https://www.abcam.com/ab49124 - only tested in Western blot application,

      https://www.abcam.com/ab108297 - Tissue culture supernatant (conc not provided); recommended starting dilution 1/250 - 1/500,

      https://www.abcam.com/ab133485 - Tissue culture supernatant (conc not provided); recommended starting dilution 1/250 - 1/500,

      https://www.abcam.com/ab16667 - Tissue culture supernatant (conc not provided); recommended starting dilution1/100



      If you need any further assistance in the future, please do not hesitate to contact me.

      Read More

      Question

      Hope this e-mail finds you well!
      Kindly advise how many tests can be performed with our below abcam products as desired by the customer :
      Cat No.
      Description
      Pack Size
      ab10403
      Mouse monoclonal [44716.111] to CXCR4
      100 µg
      ab133485
      Rabbit monoclonal [EPR4646] to FLI1
      100 µl
      ab9087
      Mouse monoclonal [MEM-131] to CD99
      100 µg
      ab1101
      Mouse monoclonal to p53
      50 µg
      Looking forward towards your valued response on it.

      With Best Regards,

      Read More
      Answer

      Thank you for contacting us.

      The number of tests totally depends on the dilution or concentration range customer uses. However at the recommended dilution, the antibody can be used for 100 ICC/IF or 100 IHC-P or 100 WB tests.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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