Recombinant Anti-FLI1 antibody [EPR4646] (ab133485)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4646] to FLI1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-FLI1 antibody [EPR4646]
See all FLI1 primary antibodies -
Description
Rabbit monoclonal [EPR4646] to FLI1 -
Host species
Rabbit -
Specificity
This antibody does not cross-react with ERG.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human FLI1 aa 350-450. The exact sequence is proprietary.
(Peptide available asab187137) -
Positive control
- U937, Jurkat, Raw264.7 and HL-60 cell lysates; Human Ewing sarcoma tissue; FLI1 recombinant protein
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4646 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/700. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified, use 1/140. |
|
WB |
1/1000. Predicted molecular weight: 51 kDa.
For unpurified, use 1/200. |
Notes |
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IHC-P
1/700. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified, use 1/140. |
WB
1/1000. Predicted molecular weight: 51 kDa. For unpurified, use 1/200. |
Target
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Function
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. -
Involvement in disease
Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2313 Human
- Entrez Gene: 14247 Mouse
- Omim: 193067 Human
- SwissProt: Q01543 Human
- SwissProt: P26323 Mouse
- Unigene: 504281 Human
- Unigene: 258908 Mouse
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Alternative names
- ERGB transcription factor antibody
- Ewing Sarcoma breakpoint region 2 antibody
- EWSR 2 antibody
see all
Images
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Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (purified) + Jurkat cell lysate at 10 µg
Secondary
HRP goat anti-rabbit at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human lung with purified ab133485 at a working dilution of 1 in 700. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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Anti-FLI1 antibody [EPR4646] (ab133485) at 1/200 dilution (unpurified) + Jurkat cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (unpurified)
Lane 1 : U937 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Raw264.7 cell lysate
Lane 4 : HL-60 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDa -
All lanes : Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (unpurified)
Lane 1 : FLI1 recombinant protein
Lane 2 : ERG
recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDa -
Immunohistochemical staining of paraffin embedded human lung with unpurified ab133485 at a working dilution of 1 in 140. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labelled with unpurified ab133485 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab133485 has been referenced in 24 publications.
- Riggan L et al. The transcription factor Fli1 restricts the formation of memory precursor NK cells during viral infection. Nat Immunol 23:556-567 (2022). PubMed: 35288713
- Buchou C et al. Upregulation of the Mevalonate Pathway through EWSR1-FLI1/EGR2 Regulatory Axis Confers Ewing Cells Exquisite Sensitivity to Statins. Cancers (Basel) 14:N/A (2022). PubMed: 35565457
- Kitagawa T et al. AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells. PLoS One 17:e0269077 (2022). PubMed: 36194562
- Hu A et al. UM171 cooperates with PIM1 inhibitors to restrict HSC expansion markers and suppress leukemia progression. Cell Death Discov 8:448 (2022). PubMed: 36335089
- Wang C et al. FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome. Front Immunol 12:607836 (2021). PubMed: 33717090