Recombinant
RabMAb

Anti-FLI1 antibody [EPR4646] (Alexa Fluor® 647) (ab196146)

Overview

  • Product name
    Anti-FLI1 antibody [EPR4646] (Alexa Fluor® 647)
    See all FLI1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4646] to FLI1 (Alexa Fluor® 647)
  • Host species
    Rabbit
  • Conjugation
    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FLI1 aa 350-450. The exact sequence is proprietary.
    (Peptide available as ab187137)

  • Positive control
    • ICC/IF: Jurkat cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

Target

  • Function
    Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
  • Involvement in disease
    Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERGB transcription factor antibody
    • Ewing Sarcoma breakpoint region 2 antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLI 1 antibody
    • FLI 1 proto oncogene antibody
    • Fli-1 proto-oncogene, ETS transcription factor antibody
    • FLI1 antibody
    • FLI1 EWS fusion gene antibody
    • FLI1 proto oncogene antibody
    • FLI1_HUMAN antibody
    • Friend leukemia integration 1 (FLI1) transcription factor antibody
    • Friend leukemia integration 1 transcription factor antibody
    • Friend leukemia virus integration 1 antibody
    • Proto-oncogene Fli-1 antibody
    • Sarcoma breakpoint region 2 (EWSR2) antibody
    • SIC 1 antibody
    • SIC1 antibody
    • Transcription factor ERGB antibody
    • Viral integration region FLI1 antibody
    • Viral integration region FLI1, mouse, homolog of antibody
    see all

Images

  • ab196146 staining FLI-1 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196146 at 1/50 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab196146 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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