Recombinant
RabMAb

Anti-FLI1 antibody [EPR4646] - BSA and Azide free (ab215987)

Overview

  • Product name
    Anti-FLI1 antibody [EPR4646] - BSA and Azide free
    See all FLI1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4646] to FLI1 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • U937, Jurkat, Raw264.7 and HL-60 cell lysates; Human Ewing sarcoma tissue; FLI1 recombinant protein
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 51 kDa.

 

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
    • Involvement in disease
      Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
    • Sequence similarities
      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ERGB transcription factor antibody
      • Ewing Sarcoma breakpoint region 2 antibody
      • EWSR 2 antibody
      • EWSR2 antibody
      • FLI 1 antibody
      • FLI 1 proto oncogene antibody
      • Fli-1 proto-oncogene, ETS transcription factor antibody
      • FLI1 antibody
      • FLI1 EWS fusion gene antibody
      • FLI1 proto oncogene antibody
      • FLI1_HUMAN antibody
      • Friend leukemia integration 1 (FLI1) transcription factor antibody
      • Friend leukemia integration 1 transcription factor antibody
      • Friend leukemia virus integration 1 antibody
      • Proto-oncogene Fli-1 antibody
      • Sarcoma breakpoint region 2 (EWSR2) antibody
      • SIC 1 antibody
      • SIC1 antibody
      • Transcription factor ERGB antibody
      • Viral integration region FLI1 antibody
      • Viral integration region FLI1, mouse, homolog of antibody
      see all

    Images

    • Immunohistochemical staining of paraffin embedded human lung with purified ab133485 at a working dilution of 1 in 700. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133485).

    • Immunohistochemical staining of paraffin embedded human lung with unpurified ab133485 at a working dilution of 1 in 140. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133485).

    • Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labelled with unpurified ab133485 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133485).

    References

    ab215987 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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