Key features and details
- Rabbit polyclonal to Flightless I
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Flightless I antibody
See all Flightless I primary antibodies
DescriptionRabbit polyclonal to Flightless I
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Human Flightless I aa 896-1176. Two N-terminal Tags. Expressed in E.coli.
LMEEWNEDLDGMEGFVLEGKKFARLPEEEFGHFYTQDCYVFLCRYWVPVE YEEEEKKEDKEEKAEGKEGEEATAEAEEKQPEEDFQCIVYFWQGREASNM GWLTFTFSLQKKFESLFPGKLEVVRMTQQQENPKFLSHFKRKFIIHRGKR KAVQGAQQPSLYQIRTNGSALCTRCIQINTDSSLLNSEFCFILKVPFESE DNQGIVYAWVGRASDPDEAKLAEDILNTMFDTSYSKQVINEGEEPENFFW VGIGAQKPYDDDAEYMKHTRLFRCSNEKGYF
Database link: Q13045-1
- WB: Recombinant human Flightless I protein; Pig kidney lysate; Human lung lysate. IHC-P: Human skeletal muscle tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionMay play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
Tissue specificityStrongest expression in skeletal muscle with high expression also in the heart and lung.
Involvement in diseaseDeletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
Sequence similaritiesContains 5 gelsolin-like repeats.
Contains 15 LRR (leucine-rich) repeats.
Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
- Information by UniProt
- Fli 1 antibody
- FLI antibody
- Fli1 antibody
All lanes : Anti-Flightless I antibody (ab233001) at 2 µg/ml
Lane 1 : Human lung tissue lysate.
Lane 2 : Pig kidney tissue lysate.
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Flightless I using ab233001 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Anti-Flightless I antibody (ab233001) at 2 µg/ml + Recombinant human Flightless I protein
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233001 has not yet been referenced specifically in any publications.