Overview

  • Product name

    Anti-Flightless I antibody - C-terminal
    See all Flightless I primary antibodies
  • Description

    Rabbit polyclonal to Flightless I - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Flightless I (C terminal). The exact sequence is proprietary.
    Database link: Q13045

  • Positive control

    • WB: Raji whole cell lysate; Mouse brain lysate; Rat muscle lysate. ICC/IF: Raji cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 145 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
  • Tissue specificity

    Strongest expression in skeletal muscle with high expression also in the heart and lung.
  • Involvement in disease

    Deletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
  • Sequence similarities

    Contains 5 gelsolin-like repeats.
    Contains 15 LRR (leucine-rich) repeats.
  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fli 1 antibody
    • FLI antibody
    • Fli1 antibody
    • Flightless 1 antibody
    • Flightless I (Drosophila) homolog antibody
    • Flightless I homolog antibody
    • Flightless I homolog (Drosophila) antibody
    • Flightless1 antibody
    • FlightlessI antibody
    • fliI antibody
    • FLII_HUMAN antibody
    • Fliih antibody
    • FLIL antibody
    • MGC39265 antibody
    • Protein flightless 1 homolog antibody
    • Protein flightless-1 homolog antibody
    see all

Images

  • Anti-Flightless I antibody - C-terminal (ab228616) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 145 kDa



    5% SDS-PAGE gel.

  • Anti-Flightless I antibody - C-terminal (ab228616) at 1/1000 dilution + Rat muscle lysate at 50 µg

    Predicted band size: 145 kDa



    5% SDS-PAGE gel.

  • Anti-Flightless I antibody - C-terminal (ab228616) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Predicted band size: 145 kDa



    5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed Raji (human Burkitt's lymphoma cell line) cells stained for Flightless I (green) using ab228616 at 1/500 dilution in ICC/IF.

    Nuclear counter stain: Hoechst 33342 (blue).

References

ab228616 has not yet been referenced specifically in any publications.

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