Recombinant
RabMAb

Anti-Flightless I antibody [EPR4201] (ab108594)

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Overview

  • Product name
    Anti-Flightless I antibody [EPR4201]
    See all Flightless I primary antibodies
  • Description
    Rabbit monoclonal [EPR4201] to Flightless I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Flightless I aa 1-100. The exact sequence is proprietary.

  • Positive control
    • HeLa, A549 and A431 cell lysates; Human skeletal muscle tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function
      May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
    • Tissue specificity
      Strongest expression in skeletal muscle with high expression also in the heart and lung.
    • Involvement in disease
      Deletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
    • Sequence similarities
      Contains 5 gelsolin-like repeats.
      Contains 15 LRR (leucine-rich) repeats.
    • Cellular localization
      Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Fli 1 antibody
      • FLI antibody
      • Fli1 antibody
      • Flightless 1 antibody
      • Flightless I (Drosophila) homolog antibody
      • Flightless I homolog antibody
      • Flightless I homolog (Drosophila) antibody
      • Flightless1 antibody
      • FlightlessI antibody
      • fliI antibody
      • FLII_HUMAN antibody
      • Fliih antibody
      • FLIL antibody
      • MGC39265 antibody
      • Protein flightless 1 homolog antibody
      • Protein flightless-1 homolog antibody
      see all

    Images

    • Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)
      Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)
      All lanes : Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 145 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (ab108594)
      Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (ab108594)
      ab108594 at 1/100 dilution staining Flightless I in HeLa cells by Immunofluorescence.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (ab108594)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (ab108594)
      ab108594 at 1/100 dilution staining Flightless I in Human skeletal muscle by Immunohistochemistry, Paraffin-embedded tissue

    References

    This product has been referenced in:
    • Arora PD  et al. Flightless I interacts with NMMIIA to promote cell extension formation, which enables collagen remodeling. Mol Biol Cell 26:2279-97 (2015). IP . Read more (PubMed: 25877872) »

    See 1 Publication for this product

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