Recombinant Anti-Flightless I antibody [EPR4201] (ab108594)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Flightless I antibody [EPR4201]
See all Flightless I primary antibodies -
Description
Rabbit monoclonal [EPR4201] to Flightless I -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Flightless I aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: HeLa, A549 and A431 cell lysates. IHC-P: Human skeletal muscle tissue. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4201 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lysates
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab108594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 145 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
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| ICC/IF | 1/100 - 1/250. |
Target
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Function
May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration. -
Tissue specificity
Strongest expression in skeletal muscle with high expression also in the heart and lung. -
Involvement in disease
Deletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems. -
Sequence similarities
Contains 5 gelsolin-like repeats.
Contains 15 LRR (leucine-rich) repeats. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes. - Information by UniProt
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Database links
- Entrez Gene: 2314 Human
- Entrez Gene: 14248 Mouse
- Entrez Gene: 287375 Rat
- Omim: 600362 Human
- SwissProt: Q13045 Human
- SwissProt: Q9JJ28 Mouse
- Unigene: 513984 Human
- Unigene: 339755 Mouse
see all -
Alternative names
- Fli 1 antibody
- FLI antibody
- Fli1 antibody
see all
Images
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![Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)](https://www.abcam.com/ps/products/108/ab108594/Images/ab108594-389431-anti-flightless-i-antibody-epr4201-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)All lanes : Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FLII knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 145 kDa
Observed band size: 145 kDaLanes 1-3: Merged signal (red and green). Green - ab108594 observed at 145 kDa. Red - loading control ab7291 observed at 50 kDa.
ab108594 Anti-Flightless I antibody [EPR4201] was shown to specifically react with Flightless in wild-type HeLa cells. Loss of signal was observed when knockout sample ab257954 was used. Wild-type and Flightless knockout samples were subjected to SDS-PAGE. ab108594 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (ab108594)](https://www.abcam.com/ps/products/108/ab108594/Images/ab108594-121283-anti-flightless-i-antibody-epr4201-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (ab108594)ab108594 at 1/100 dilution staining Flightless I in HeLa cells by Immunofluorescence. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (ab108594)](https://www.abcam.com/ps/products/108/ab108594/Images/ab108594-121282-anti-flightless-i-antibody-epr4201-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (ab108594)ab108594 at 1/100 dilution staining Flightless I in Human skeletal muscle by Immunohistochemistry, Paraffin-embedded tissue
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)](https://www.abcam.com/ps/products/108/ab108594/Images/ab108594-121284-anti-flightless-i-antibody-epr4201-western-blot.gif)
Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)All lanes : Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 145 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Arora PD et al. Flightless I interacts with NMMIIA to promote cell extension formation, which enables collagen remodeling. Mol Biol Cell 26:2279-97 (2015). IP . Read more (PubMed: 25877872) »
