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  1. Link

    flightless-i-antibody-epr4201-ab108594.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Flightless I antibody [EPR4201] (ab108594)

  • Datasheet
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Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)
  • Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (ab108594)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (ab108594)
  • Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)
  • Anti-Flightless I antibody [EPR4201] (ab108594)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4201] to Flightless I
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Knockout
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Human FLII (Flightless I) knockout HeLa cell line (ab265890)

View more associated products

Overview

  • Product name

    Anti-Flightless I antibody [EPR4201]
    See all Flightless I primary antibodies
  • Description

    Rabbit monoclonal [EPR4201] to Flightless I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, A549 and A431 cell lysates. IHC-P: Human skeletal muscle tissue. ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4201
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Stem Cells
    • Mesenchymal Stem Cells
    • Intracellular

Associated products

  • Alternative Versions

    • Anti-Flightless I antibody [EPR4201] - BSA and Azide free (ab247692)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human FLII (Flightless I) knockout HeLa cell line (ab265890)
  • KO cell lysates

    • Human FLII (Flightless I) knockout HeLa cell lysate (ab257954)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
  • Tissue specificity

    Strongest expression in skeletal muscle with high expression also in the heart and lung.
  • Involvement in disease

    Deletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
  • Sequence similarities

    Contains 5 gelsolin-like repeats.
    Contains 15 LRR (leucine-rich) repeats.
  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
  • Target information above from: UniProt accession Q13045 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2314 Human
    • Entrez Gene: 14248 Mouse
    • Entrez Gene: 287375 Rat
    • Omim: 600362 Human
    • SwissProt: Q13045 Human
    • SwissProt: Q9JJ28 Mouse
    • Unigene: 513984 Human
    • Unigene: 339755 Mouse
    • Alternative names

      • Fli 1 antibody
      • FLI antibody
      • Fli1 antibody
      • Flightless 1 antibody
      • Flightless I (Drosophila) homolog antibody
      • Flightless I homolog antibody
      • Flightless I homolog (Drosophila) antibody
      • Flightless1 antibody
      • FlightlessI antibody
      • fliI antibody
      • FLII_HUMAN antibody
      • Fliih antibody
      • FLIL antibody
      • MGC39265 antibody
      • Protein flightless 1 homolog antibody
      • Protein flightless-1 homolog antibody
      see all

    Images

    • Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)
      Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)
      All lanes : Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : FLII knockout HeLa cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 145 kDa
      Observed band size: 145 kDa



      Lanes 1-3: Merged signal (red and green). Green - ab108594 observed at 145 kDa. Red - loading control ab7291 observed at 50 kDa.

      ab108594 Anti-Flightless I antibody [EPR4201] was shown to specifically react with Flightless I in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265890 (knockout cell lysate ab257954) was used. Wild-type and Flightless I knockout samples were subjected to SDS-PAGE. ab108594 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (ab108594)
      Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] (ab108594)
      ab108594 at 1/100 dilution staining Flightless I in HeLa cells by Immunofluorescence.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (ab108594)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] (ab108594)

      ab108594 at 1/100 dilution staining Flightless I in Human skeletal muscle by Immunohistochemistry, Paraffin-embedded tissue

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)
      Western blot - Anti-Flightless I antibody [EPR4201] (ab108594)
      All lanes : Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 145 kDa

    • Anti-Flightless I antibody [EPR4201] (ab108594)
      Anti-Flightless I antibody [EPR4201] (ab108594)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab108594? Please let us know so that we can cite the reference in this datasheet.

    ab108594 has been referenced in 1 publication.

    • Arora PD  et al. Flightless I interacts with NMMIIA to promote cell extension formation, which enables collagen remodeling. Mol Biol Cell 26:2279-97 (2015). IP . PubMed: 25877872

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