Product nameAnti-Flightless I antibody [EPR4202(2)]
See all Flightless I primary antibodies
DescriptionRabbit monoclonal [EPR4202(2)] to Flightless I
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Flightless I aa 1200-1300 (internal sequence). The exact sequence is proprietary.
- Human heart, HeLa, A431, and A549 lysates; Human muscle tissue; HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 145 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionMay play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
Tissue specificityStrongest expression in skeletal muscle with high expression also in the heart and lung.
Involvement in diseaseDeletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
Sequence similaritiesContains 5 gelsolin-like repeats.
Contains 15 LRR (leucine-rich) repeats.
Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
- Information by UniProt
- Fli 1 antibody
- FLI antibody
- Fli1 antibody
All lanes : Anti-Flightless I antibody [EPR4202(2)] (ab109015) at 1/1000 dilution
Lane 1 : Humam heart lysate
Lane 2 : HeLa lysate
Lane 3 : A431 lysate
Lane 4 : A549 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 145 kDa
Immunohistochemical analysis of Flightless 1 expression in paraffin-embedded Human muscle tissue using ab109015 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent staining of Flightless I in HeLa cells using ab109015 at 1/100 dilution.
ab109015 has not yet been referenced specifically in any publications.