Overview

  • Product name

  • Description

    Rabbit polyclonal to FLIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human FLIP (internal sequence). The exact sequence is proprietary.
    Database link: O15519

  • Positive control

    • WB: A431 whole cell extract. IHC-P: Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.

Target

  • Function

    Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
  • Tissue specificity

    Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • Domain

    The caspase domain lacks the active sites residues involved in catalysis.
  • Post-translational
    modifications

    Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
  • Information by UniProt
  • Database links

  • Alternative names

    • c FLIP antibody
    • c FLIPL antibody
    • c FLIPR antibody
    • c FLIPS antibody
    • c-FLIP antibody
    • c-FLIPL antibody
    • c-FLIPR antibody
    • c-FLIPS antibody
    • CASH antibody
    • CASP8 and FADD like apoptosis regulator antibody
    • CASP8 and FADD-like apoptosis regulator subunit p12 antibody
    • CASP8AP1 antibody
    • Caspase homolog antibody
    • Caspase like apoptosis regulatory protein antibody
    • Caspase related inducer of apoptosis antibody
    • Caspase-eight-related protein antibody
    • Caspase-like apoptosis regulatory protein antibody
    • Casper antibody
    • Cellular FLICE-like inhibitory protein antibody
    • CFLA antibody
    • Cflar antibody
    • CFLAR_HUMAN antibody
    • CLARP antibody
    • FADD like anti apoptotic molecule antibody
    • FADD-like antiapoptotic molecule 1 antibody
    • FLAME antibody
    • FLAME-1 antibody
    • FLAME1 antibody
    • FLIP antibody
    • I FLICE antibody
    • I-FLICE antibody
    • Inhibitor of FLICE antibody
    • MACH-related inducer of toxicity antibody
    • MRIT antibody
    • OTTHUMP00000163715 antibody
    • OTTHUMP00000206475 antibody
    • OTTHUMP00000206476 antibody
    • OTTHUMP00000206478 antibody
    • OTTHUMP00000206479 antibody
    • OTTHUMP00000206480 antibody
    • OTTHUMP00000206482 antibody
    • OTTHUMP00000207360 antibody
    • Usurpin antibody
    • Usurpin beta antibody
    see all

Images

  • Anti-FLIP antibody (ab227194) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 55 kDa



    10% SDS-PAGE

  • Paraffin embedded human colon carcinoma tissue stained for FLIP with ab227194 (1/500 dilution) in immunohistochemical analysis.

References

ab227194 has not yet been referenced specifically in any publications.

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