Key features and details
- Rabbit polyclonal to FLIP
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-FLIP antibody
See all FLIP primary antibodies
DescriptionRabbit polyclonal to FLIP
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment (His-T7-tag) corresponding to Rat FLIP aa 43-281. (Expressed in E.coli).
RDLLDCLSERGQLSFAALAELLYRVRRFDLLKRILKTDKAAVEDHLCRSP HLVSDYRVLLMEIGENLNQSDVSSLIFLTKDYTGRGKVAKDKSFLDLVIE LEKLNLIGSDQLNLLEKCLKSIHRIDLKTKIQKYTQSSQEARSNMNALQA SLPKLSIKEHLYNSRLQNGRSKEPRFVEHHGIQRKPVKTSIQESGIFLPP HIHEESYRMQSKPLGICLIIDCIGNDTEYLRETFTSLGY
Database link: C0H5Y5
- WB: Recombinant rat FLIP protein; Jurkat, HeLa and K562 cell lysates; Rat spleen lysate. IHC-P: Rat liver tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233230 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa.|
FunctionApoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Tissue specificityWidely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
DomainThe caspase domain lacks the active sites residues involved in catalysis.
modificationsProteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
- Information by UniProt
- c FLIP antibody
- c FLIPL antibody
- c FLIPR antibody
Paraffin-embedded rat liver tissue stained for FLIP using ab233230 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Anti-FLIP antibody (ab233230) at 3 µg/ml + Recombinant rat FLIP protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa
All lanes : Anti-FLIP antibody (ab233230) at 3 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : Rat spleen lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233230 has not yet been referenced specifically in any publications.