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    flip-antibody-ab8421.pdf

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Cell Biology Apoptosis Extracellular Signals Death Ligands
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Anti-FLIP antibody (ab8421)

  • Datasheet
  • SDS
Reviews (11)Q&A (9)References (15)

Product price, shipping and contact information

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Western blot - Anti-FLIP antibody (ab8421)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (ab8421)
  • Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab8421)

Key features and details

  • Rabbit polyclonal to FLIP
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human FLIP protein (denatured) (ab111628)
Primary
Product image
Anti-Caspase-10/CASP-10 antibody (ab2012)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-FLIP antibody
    See all FLIP primary antibodies
  • Description

    Rabbit polyclonal to FLIP
  • Host species

    Rabbit
  • Specificity

    Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human FLIP aa 2-19.
    Sequence:

    SAEVIHQVEEALDTDEKE


    (Peptide available as ab8457)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    FLIP Antibody is affinity chromatography purified via peptide column.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Light chain type

    unknown
  • Research areas

    • Cell Biology
    • Apoptosis
    • Extracellular Signals
    • Death Ligands
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Necroptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human FLIP protein (denatured) (ab111628)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Mouse
WB
Human
Application Abreviews Notes
WB (10)
1/500 - 1/1000. Predicted molecular weight: 55 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 55 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.

Target

  • Function

    Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
  • Tissue specificity

    Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • Domain

    The caspase domain lacks the active sites residues involved in catalysis.
  • Post-translational
    modifications

    Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
  • Target information above from: UniProt accession O15519 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8837 Human
    • Entrez Gene: 12633 Mouse
    • Omim: 603599 Human
    • SwissProt: O15519 Human
    • SwissProt: O35732 Mouse
    • Unigene: 390736 Human
    • Unigene: 336848 Mouse
    • Alternative names

      • c FLIP antibody
      • c FLIPL antibody
      • c FLIPR antibody
      • c FLIPS antibody
      • c-FLIP antibody
      • c-FLIPL antibody
      • c-FLIPR antibody
      • c-FLIPS antibody
      • CASH antibody
      • CASP8 and FADD like apoptosis regulator antibody
      • CASP8 and FADD-like apoptosis regulator subunit p12 antibody
      • CASP8AP1 antibody
      • Caspase homolog antibody
      • Caspase like apoptosis regulatory protein antibody
      • Caspase related inducer of apoptosis antibody
      • Caspase-eight-related protein antibody
      • Caspase-like apoptosis regulatory protein antibody
      • Casper antibody
      • cellular flice inhibitory protein antibody
      • Cellular FLICE-like inhibitory protein antibody
      • CFLA antibody
      • Cflar antibody
      • CFLAR_HUMAN antibody
      • CLARP antibody
      • FADD like anti apoptotic molecule antibody
      • FADD-like antiapoptotic molecule 1 antibody
      • FLAME antibody
      • FLAME-1 antibody
      • FLAME1 antibody
      • FLIP antibody
      • I FLICE antibody
      • I-FLICE antibody
      • Inhibitor of FLICE antibody
      • MACH-related inducer of toxicity antibody
      • MRIT antibody
      • OTTHUMP00000163715 antibody
      • OTTHUMP00000206475 antibody
      • OTTHUMP00000206476 antibody
      • OTTHUMP00000206478 antibody
      • OTTHUMP00000206479 antibody
      • OTTHUMP00000206480 antibody
      • OTTHUMP00000206482 antibody
      • OTTHUMP00000207360 antibody
      • Usurpin antibody
      • Usurpin beta antibody
      see all

    Images

    • Western blot - Anti-FLIP antibody (ab8421)
      Western blot - Anti-FLIP antibody (ab8421)
      Lane 1 : Anti-FLIP antibody (ab8421) at 1 µg/ml
      Lane 2 : Anti-FLIP antibody (ab8421) at 2 µg/ml

      All lanes : K562 cell lysate

      Predicted band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (ab8421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (ab8421)

      Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/ml.

    • Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab8421)
      Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab8421)

      ab8421 at 5µg/ml staining FLIP in Hela cells by ICC/IF 

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (15)

    Publishing research using ab8421? Please let us know so that we can cite the reference in this datasheet.

    ab8421 has been referenced in 15 publications.

    • Ke C  et al. Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib. Cancers (Basel) 12:N/A (2020). PubMed: 32375399
    • Dang Q  et al. The ?-secretase inhibitor GSI-I interacts synergistically with the proteasome inhibitor bortezomib to induce ALK+ anaplastic large cell lymphoma cell apoptosis. Cell Signal 59:76-84 (2019). PubMed: 30878517
    • Hata T  et al. GABA, ?-Aminobutyric Acid, Protects Against Severe Liver Injury. J Surg Res 236:172-183 (2019). PubMed: 30694753
    • Zong H  et al. miR-125b suppresses cellular proliferation by targeting c-FLIP in gallbladder carcinoma. Oncol Lett 18:6822-6828 (2019). PubMed: 31788125
    • Zhang C  et al. Identification and validation of microRNAs and their targets expressed in osteosarcoma. Oncol Lett 18:5628-5636 (2019). PubMed: 31656545
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 20 Abreviews or Q&A

    Question

    Dear xxxx
    Thank you very much for your reply. I would like to receive the blocking
    peptide of the antibody to confirm that the band of 50kDa corresponding to
    c-FLIPL. The sending of the peptide would be free? What would be the
    delivery time? Delivery would be through Tecnolab?
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 10 2012

    Answer

    Thank you for getting back to me.

    The peptide would be free, and it would be sent through Tecnolab. As it will be delivered by them I am unsure of how long it will take. The peptide is in stock and would be sent out as soon as possible. I will contact Tecnolab to enquire how long this will take to get to you.

    If you have any further questions, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Apr 10 2012

    Western blot abreview for Anti-FLIP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (B16 cells lysate)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Glycin gel, PVDF membrane)
    Loading amount
    40 µg
    Specification
    B16 cells lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 16 2019

    Western blot abreview for Anti-FLIP antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (BHK)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    40 µg
    Specification
    BHK
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Simon Boudreault

    Verified customer

    Submitted May 30 2018

    Western blot abreview for Anti-FLIP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Kasumi-1 AML cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin)
    Loading amount
    80000 cells
    Specification
    Kasumi-1 AML cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 25 2017

    Western blot abreview for Anti-FLIP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
    Loading amount
    0.5 µg
    Specification
    Rabbit Reticulocyte
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 02 2016

    Western blot abreview for Anti-FLIP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    500000 cells
    Specification
    Canine Kidney Epithelial MDCK cells
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 11 2016

    Immunoprecipitation abreview for Anti-FLIP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (A549 (human lung epithelium cells))
    Total protein in input
    1e+007 cells
    Immuno-precipitation step
    Other - Protein G Dynabeads
    Specification
    A549 (human lung epithelium cells)
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 05 2016

    Western blot abreview for Anti-FLIP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (VERO E6)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    75000 cells
    Specification
    VERO E6
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 22 2016

    Western blot abreview for Anti-FLIP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549 (human lung epithelium cells))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    75000 cells
    Specification
    A549 (human lung epithelium cells)
    Blocking step
    No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 13 2016

    Western blot abreview for Anti-FLIP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts (MEFs))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    25000 cells
    Specification
    Mouse embryonic fibroblasts (MEFs)
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 23 2016

    1-10 of 20 Abreviews or Q&A

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