Anti-FLIP antibody (ab8421)

Product name

Anti-FLIP antibody
See all FLIP primary antibodies
Description

Rabbit polyclonal to FLIP
Host species

Rabbit
Specificity

Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta.
Tested applications

Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide corresponding to Human FLIP aa 2-19.
Sequence:
SAEVIHQVEEALDTDEKE

(Peptide available as ab8457)

Run BLAST with Run BLAST with

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Concentration information loading...
Purity

Affinity purified
Purification notes

FLIP Antibody is affinity chromatography purified via peptide column.
Clonality

Polyclonal
Isotype

IgG
Light chain type

unknown
Research areas

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit (ab102918)
- APC Conjugation Kit (ab201807)
Immunizing Peptide (Blocking)
- Human FLIP peptide (ab8457)

Our Abpromise guarantee covers the use of ab8421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/500 - 1/1000. Predicted molecular weight: 55 kDa.Can be blocked with Human FLIP peptide (ab8457).
ICC/IF		Use a concentration of 5 µg/ml.
Flow Cyt		Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P		Use a concentration of 5 µg/ml.

Function

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Tissue specificity

Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
Sequence similarities

Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
Domain

The caspase domain lacks the active sites residues involved in catalysis.
Post-translational
modifications

Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
Target information above from: UniProt accession O15519 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 8837 Human
- Entrez Gene: 12633 Mouse
- Entrez Gene: 117279 Rat
- Omim: 603599 Human
- SwissProt: O15519 Human
- SwissProt: O35732 Mouse
- Unigene: 390736 Human
- Unigene: 336848 Mouse
Alternative names
- c FLIP antibody
- c FLIPL antibody
- c FLIPR antibody
- c FLIPS antibody
- c-FLIP antibody
- c-FLIPL antibody
- c-FLIPR antibody
- c-FLIPS antibody
- CASH antibody
- CASP8 and FADD like apoptosis regulator antibody
- CASP8 and FADD-like apoptosis regulator subunit p12 antibody
- CASP8AP1 antibody
- Caspase homolog antibody
- Caspase like apoptosis regulatory protein antibody
- Caspase related inducer of apoptosis antibody
- Caspase-eight-related protein antibody
- Caspase-like apoptosis regulatory protein antibody
- Casper antibody
- Cellular FLICE-like inhibitory protein antibody
- CFLA antibody
- Cflar antibody
- CFLAR_HUMAN antibody
- CLARP antibody
- FADD like anti apoptotic molecule antibody
- FADD-like antiapoptotic molecule 1 antibody
- FLAME antibody
- FLAME-1 antibody
- FLAME1 antibody
- FLIP antibody
- I FLICE antibody
- I-FLICE antibody
- Inhibitor of FLICE antibody
- MACH-related inducer of toxicity antibody
- MRIT antibody
- OTTHUMP00000163715 antibody
- OTTHUMP00000206475 antibody
- OTTHUMP00000206476 antibody
- OTTHUMP00000206478 antibody
- OTTHUMP00000206479 antibody
- OTTHUMP00000206480 antibody
- OTTHUMP00000206482 antibody
- OTTHUMP00000207360 antibody
- Usurpin antibody
- Usurpin beta antibody
see all

Western blot - Anti-FLIP antibody (ab8421)

Lane 1 : Anti-FLIP antibody (ab8421) at 1 µg/ml
Lane 2 : Anti-FLIP antibody (ab8421) at 2 µg/ml

All lanes : K562 cell lysate

Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (ab8421)

Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/ml.
Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab8421)

ab8421 at 5µg/ml staining FLIP in Hela cells by ICC/IF

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

This product has been referenced in:

Padmanabhan C et al. cFLIP critically modulates apoptotic resistance in epithelial-to-mesenchymal transition. Oncotarget 8:101072-101086 (2017). Read more (PubMed: 29254146) »
Hernandez L et al. A dual role for Caspase8 and NF-?B interactions in regulating apoptosis and necroptosis of ovarian cancer, with correlation to patient survival. Cell Death Discov 1:15053 (2015). WB . Read more (PubMed: 28179987) »

See all 9 Publications for this product

Publishing research using ab8421? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 19 Abreviews or Q&A

Question

Dear xxxx
Thank you very much for your reply. I would like to receive the blocking
peptide of the antibody to confirm that the band of 50kDa corresponding to
c-FLIPL. The sending of the peptide would be free? What would be the
delivery time? Delivery would be through Tecnolab?
Best regards,

Abcam community

Verified customer

Asked on Apr 10 2012

Answer

Thank you for getting back to me.

The peptide would be free, and it would be sent through Tecnolab. As it will be delivered by them I am unsure of how long it will take. The peptide is in stock and would be sent out as soon as possible. I will contact Tecnolab to enquire how long this will take to get to you.

If you have any further questions, please do not hesitate to contact us again.

Abcam Scientific Support

Answered on Apr 10 2012

Abcam has not validated the combination of species/application used in this Abreview.

Application

Western blot

Sample

Hamster Cell lysate - whole cell (BHK)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

40 µg

Specification

BHK

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Simon Boudreault

Verified customer

Submitted May 30 2018

Application

Western blot

Sample

Human Cell lysate - nuclear (Kasumi-1 AML cells)

Gel Running Conditions

Reduced Denaturing (4-20% Tris Glycin)

Loading amount

80000 cells

Specification

Kasumi-1 AML cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 25 2017

Abcam has not validated the combination of species/application used in this Abreview.

Application

Western blot

Sample

Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))

Loading amount

0.5 µg

Specification

Rabbit Reticulocyte

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 02 2016

Abcam has not validated the combination of species/application used in this Abreview.

Application

Western blot

Sample

Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)

Loading amount

500000 cells

Specification

Canine Kidney Epithelial MDCK cells

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 11 2016

Abcam has not validated the combination of species/application used in this Abreview.

Application

Immunoprecipitation

Sample

Human Cell lysate - whole cell (A549 (human lung epithelium cells))

Total protein in input

1e+007 cells

Immuno-precipitation step

Other - Protein G Dynabeads

Specification

A549 (human lung epithelium cells)

Abcam user community

Verified customer

Submitted Nov 05 2016

Application

Western blot

Sample

African Green Monkey Cell lysate - whole cell (VERO E6)

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)

Loading amount

75000 cells

Specification

VERO E6

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 22 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (A549 (human lung epithelium cells))

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)

Loading amount

75000 cells

Specification

A549 (human lung epithelium cells)

Blocking step

No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 13 2016

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts (MEFs))

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)

Loading amount

25000 cells

Specification

Mouse embryonic fibroblasts (MEFs)

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 23 2016

Application

Western blot

Loading amount

25 µg

Gel Running Conditions

Reduced Denaturing

Sample

Mouse Cell lysate - whole cell (liver, hepatocytes)

Specification

liver, hepatocytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 05 2015

1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-FLIP antibody (ab8421)

Overview

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References

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Customer reviews and Q&As

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Western blot abreview for Anti-FLIP antibody

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