Anti-FLIP antibody (ab8421)
Key features and details
- Rabbit polyclonal to FLIP
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FLIP antibody
See all FLIP primary antibodies -
Description
Rabbit polyclonal to FLIP -
Host species
Rabbit -
Specificity
Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta. -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human FLIP aa 2-19.
Sequence:SAEVIHQVEEALDTDEKE
(Peptide available asab8457) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
FLIP Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (10) |
1/500 - 1/1000. Predicted molecular weight: 55 kDa.
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| IHC-P |
Use a concentration of 5 µg/ml.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 55 kDa. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. -
Tissue specificity
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
Domain
The caspase domain lacks the active sites residues involved in catalysis. -
Post-translational
modificationsProteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. - Information by UniProt
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Database links
- Entrez Gene: 8837 Human
- Entrez Gene: 12633 Mouse
- Entrez Gene: 117279 Rat
- Omim: 603599 Human
- SwissProt: O15519 Human
- SwissProt: O35732 Mouse
- Unigene: 390736 Human
- Unigene: 336848 Mouse
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Alternative names
- c FLIP antibody
- c FLIPL antibody
- c FLIPR antibody
see all
Images
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Western blot - Anti-FLIP antibody (ab8421)Lane 1 : Anti-FLIP antibody (ab8421) at 1 µg/ml
Lane 2 : Anti-FLIP antibody (ab8421) at 2 µg/ml
All lanes : K562 cell lysate
Predicted band size: 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (ab8421)Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab8421)ab8421 at 5µg/ml staining FLIP in Hela cells by ICC/IF
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab8421 has been referenced in 18 publications.
- Ke C et al. Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib. Cancers (Basel) 12:N/A (2020). PubMed: 32375399
- Liu YP et al. circPVT1 Facilitates Invasion and Metastasis by Regulating miR-205-5p/c-FLIP Axis in Osteosarcoma. Cancer Manag Res 12:1229-1240 (2020). PubMed: 32110097
- Maiese A et al. Hypo-Expression of Flice-Inhibitory Protein and Activation of the Caspase-8 Apoptotic Pathways in the Death-Inducing Signaling Complex Due to Ischemia Induced by the Compression of the Asphyxiogenic Tool on the Skin in Hanging Cases. Diagnostics (Basel) 10:N/A (2020). PubMed: 33198065
- Ariana A et al. Tristetraprolin regulates necroptosis during tonic Toll-like receptor 4 (TLR4) signaling in murine macrophages. J Biol Chem 295:4661-4672 (2020). PubMed: 32094226
- Dang Q et al. The ?-secretase inhibitor GSI-I interacts synergistically with the proteasome inhibitor bortezomib to induce ALK+ anaplastic large cell lymphoma cell apoptosis. Cell Signal 59:76-84 (2019). PubMed: 30878517