Anti-FLIP antibody (ab8421)
Key features and details
- Rabbit polyclonal to FLIP
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FLIP antibody
See all FLIP primary antibodies -
Description
Rabbit polyclonal to FLIP -
Host species
Rabbit -
Specificity
Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseWB Human
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Immunogen
Synthetic peptide corresponding to Human FLIP aa 2-19.
Sequence:SAEVIHQVEEALDTDEKE
(Peptide available asab8457)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
FLIP Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Mouse
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (10) |
1/500 - 1/1000. Predicted molecular weight: 55 kDa.
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| IHC-P |
Use a concentration of 5 µg/ml.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 55 kDa. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. -
Tissue specificity
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
Domain
The caspase domain lacks the active sites residues involved in catalysis. -
Post-translational
modificationsProteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. - Information by UniProt
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Database links
- Entrez Gene: 8837 Human
- Entrez Gene: 12633 Mouse
- Omim: 603599 Human
- SwissProt: O15519 Human
- SwissProt: O35732 Mouse
- Unigene: 390736 Human
- Unigene: 336848 Mouse
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Alternative names
- c FLIP antibody
- c FLIPL antibody
- c FLIPR antibody
see all
Images
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Western blot - Anti-FLIP antibody (ab8421)Lane 1 : Anti-FLIP antibody (ab8421) at 1 µg/ml
Lane 2 : Anti-FLIP antibody (ab8421) at 2 µg/ml
All lanes : K562 cell lysate
Predicted band size: 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (ab8421)Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab8421)ab8421 at 5µg/ml staining FLIP in Hela cells by ICC/IF
Protocols
References (15)
ab8421 has been referenced in 15 publications.
- Ke C et al. Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib. Cancers (Basel) 12:N/A (2020). PubMed: 32375399
- Dang Q et al. The ?-secretase inhibitor GSI-I interacts synergistically with the proteasome inhibitor bortezomib to induce ALK+ anaplastic large cell lymphoma cell apoptosis. Cell Signal 59:76-84 (2019). PubMed: 30878517
- Hata T et al. GABA, ?-Aminobutyric Acid, Protects Against Severe Liver Injury. J Surg Res 236:172-183 (2019). PubMed: 30694753
- Zong H et al. miR-125b suppresses cellular proliferation by targeting c-FLIP in gallbladder carcinoma. Oncol Lett 18:6822-6828 (2019). PubMed: 31788125
- Zhang C et al. Identification and validation of microRNAs and their targets expressed in osteosarcoma. Oncol Lett 18:5628-5636 (2019). PubMed: 31656545
