Overview

  • Product name
    Anti-FLIP antibody
    See all FLIP primary antibodies
  • Description
    Rabbit polyclonal to FLIP
  • Host species
    Rabbit
  • Specificity
    Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta.
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human FLIP aa 2-19.
    Sequence:

    SAEVIHQVEEALDTDEKE


    (Peptide available as ab8457)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab8421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.Can be blocked with Human FLIP peptide (ab8457).
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
  • Tissue specificity
    Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • Domain
    The caspase domain lacks the active sites residues involved in catalysis.
  • Post-translational
    modifications
    Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
  • Information by UniProt
  • Database links
    • Alternative names
      • c FLIP antibody
      • c FLIPL antibody
      • c FLIPR antibody
      • c FLIPS antibody
      • c-FLIP antibody
      • c-FLIPL antibody
      • c-FLIPR antibody
      • c-FLIPS antibody
      • CASH antibody
      • CASP8 and FADD like apoptosis regulator antibody
      • CASP8 and FADD-like apoptosis regulator subunit p12 antibody
      • CASP8AP1 antibody
      • Caspase homolog antibody
      • Caspase like apoptosis regulatory protein antibody
      • Caspase related inducer of apoptosis antibody
      • Caspase-eight-related protein antibody
      • Caspase-like apoptosis regulatory protein antibody
      • Casper antibody
      • Cellular FLICE-like inhibitory protein antibody
      • CFLA antibody
      • Cflar antibody
      • CFLAR_HUMAN antibody
      • CLARP antibody
      • FADD like anti apoptotic molecule antibody
      • FADD-like antiapoptotic molecule 1 antibody
      • FLAME antibody
      • FLAME-1 antibody
      • FLAME1 antibody
      • FLIP antibody
      • I FLICE antibody
      • I-FLICE antibody
      • Inhibitor of FLICE antibody
      • MACH-related inducer of toxicity antibody
      • MRIT antibody
      • OTTHUMP00000163715 antibody
      • OTTHUMP00000206475 antibody
      • OTTHUMP00000206476 antibody
      • OTTHUMP00000206478 antibody
      • OTTHUMP00000206479 antibody
      • OTTHUMP00000206480 antibody
      • OTTHUMP00000206482 antibody
      • OTTHUMP00000207360 antibody
      • Usurpin antibody
      • Usurpin beta antibody
      see all

    Images

    • Western blot - Anti-FLIP antibody (ab8421)
      Western blot - Anti-FLIP antibody (ab8421)
      Lane 1 : Anti-FLIP antibody (ab8421) at 1 µg/ml
      Lane 2 : Anti-FLIP antibody (ab8421) at 2 µg/ml

      All lanes : K562 cell lysate

      Predicted band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (ab8421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (ab8421)

      Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/ml.

    • Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab8421)
      Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab8421)

      ab8421 at 5µg/ml staining FLIP in Hela cells by ICC/IF 

    References

    This product has been referenced in:
    • Padmanabhan C  et al. cFLIP critically modulates apoptotic resistance in epithelial-to-mesenchymal transition. Oncotarget 8:101072-101086 (2017). Read more (PubMed: 29254146) »
    • Hernandez L  et al. A dual role for Caspase8 and NF-?B interactions in regulating apoptosis and necroptosis of ovarian cancer, with correlation to patient survival. Cell Death Discov 1:15053 (2015). WB . Read more (PubMed: 28179987) »

    See all 9 Publications for this product

    Publishing research using ab8421? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    19 reviews or Q&A

    10
    Votes: Highest First

    Dear xxxx
    Thank you very much for your reply. I would like to receive the blocking
    peptide of the antibody to confirm that the band of 50kDa corresponding to
    c-FLIPL. The sending of the peptide would be free? What would be the
    deliv...

    Read More

    Thank you for getting back to me.

    The peptide would be free, and it would be sent through Tecnolab. As it will be delivered by them I am unsure of how long it will take. The peptide is in stock and would be sent out as soon as possible. I wil...

    Read More

    Western blot abreview for Anti-FLIP antibody

     Below Average
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (BHK)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    40 µg
    Specification
    BHK
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
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    Username

    Simon Boudreault

    Verified customer

    Submitted May 30 2018

    Western blot abreview for Anti-FLIP antibody

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Kasumi-1 AML cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin)
    Loading amount
    80000 cells
    Specification
    Kasumi-1 AML cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Oct 25 2017

    Western blot abreview for Anti-FLIP antibody

     Good
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
    Loading amount
    0.5 µg
    Specification
    Rabbit Reticulocyte
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Dec 02 2016

    Western blot abreview for Anti-FLIP antibody

     Good
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    500000 cells
    Specification
    Canine Kidney Epithelial MDCK cells
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Nov 11 2016

    Immunoprecipitation abreview for Anti-FLIP antibody

     Good
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (A549 (human lung epithelium cells))
    Total protein in input
    1e+007 cells
    Immuno-precipitation step
    Other - Protein G Dynabeads
    Specification
    A549 (human lung epithelium cells)
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Nov 05 2016

    Western blot abreview for Anti-FLIP antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (VERO E6)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    75000 cells
    Specification
    VERO E6
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Jul 22 2016

    Western blot abreview for Anti-FLIP antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549 (human lung epithelium cells))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    75000 cells
    Specification
    A549 (human lung epithelium cells)
    Blocking step
    No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jul 13 2016

    Western blot abreview for Anti-FLIP antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts (MEFs))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    25000 cells
    Specification
    Mouse embryonic fibroblasts (MEFs)
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted May 23 2016

    Western blot abreview for Anti-FLIP antibody

     Good
    Abreviews
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (liver, hepatocytes)
    Specification
    liver, hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jan 05 2015

    1-10 of 19 Abreviews or Q&A

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