Western blot abreview for Anti-FLIP antibody

Good
Abreviews
Application
Western blot
Sample
Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
500000 cells
Specification
Canine Kidney Epithelial MDCK cells
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Cor Odyssey Blocking Buffer (TBS)
Dilution
1/1000

Secondary antibody

Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Near-Infrared Fluorescence Imaging (NIR)
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 50, 55, 57 kDa Non-specific: 36, 100, 120 kDa

Additional data

Additional Notes
Both anti-FLIP antibody (ab8421) and anti-beta actin (ab6276) antibodies were used for the western blot. The Anti-FLIP antibody (ab8421) itself detected specific bands at 50, 55, 57 kDa (green bands). The 42 kDa red band is the beta actin band (red band).

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Submitted Nov 11 2016

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