Western blot abreview for Anti-FLIP antibody

Good
Abreviews
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (VERO E6)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
75000 cells
Specification
VERO E6
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Cor Odyssey Blocking Buffer (TBS)
Dilution
1/2000

Secondary antibody

Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye 680RD Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680

Detection

Detection method
Near-Infrared Fluorescence Imaging (NIR)
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 25, 37, 48, 60 kDa Non-specific: 90, 105, 160, 200 kDa

Additional data

Additional Notes
The immunogen of FLIP antibody is synthetic peptide corresponding to Human FLIP aa 2-19 which is 100% conserved in all FLIP variants of African Green Monkey. Therefore the FLIP antibody should detect African Green Monkey's FLIP proteins. Both anti-FLIP and anti-beta actin (ab6276) antibodies were used for the western blot. The 42-43 kDa green band is the beta actin band. The anti-FLIP antibody itself detected various FLIP proteins ranging from 25-60 kDa (red bands). Some unspecific bands can be detected in the high molecular weight region.

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Submitted Jul 22 2016

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