Immunoprecipitation abreview for Anti-FLIP antibody

Good
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (A549 (human lung epithelium cells))
Total protein in input
1e+007 cells
Immuno-precipitation step
Other - Protein G Dynabeads
Specification
A549 (human lung epithelium cells)

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Pierce™ IP Lysis Buffer
Dilution
1/500

Western Blot antibody

Dilution
1/1000
Name
Abcam antibody:Anti-FLIP antibody

Additional data

Additional Notes
Lane 1: IP by control rabbit IgG (Abcam ab27472) Lane 2: IP by FLIP antibody (Abcam ab8421). 2ug of each antibody was used for IP experiments. Bands corresponding to FLIP (36-50kDa) can be detected in FLIP antibody pulldown sample but not control IgG sample.

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Submitted Nov 05 2016

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