Western blot abreview for Anti-FLIP antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (Kasumi-1 AML cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin)
Loading amount
80000 cells
Specification
Kasumi-1 AML cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody #7074
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Clarity Western ECL in Li-COR Cdigit
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 55 kDa

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Submitted Oct 25 2017

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