Western blot abreview for Anti-FLIP antibody

Below Average
Abreviews
Application
Western blot
Sample
Hamster Cell lysate - whole cell (BHK)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
BHK
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
15 hour(s) and 40 minute(s) · Temperature: 4°C · Diluent: 2,5% milk in PBS 1x
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
NA
Exposure
15 second(s)
Bands
Specific: 55, 30 kDa Non-specific: 160, 24, 25, 23, 55, 47, 4 kDa
Positive control
NA

Additional data

Additional Notes
Lane 1 = 40µg BHK cell lysate
Abcam response
We would like to thank the reviewer for submitting an AbReview for this product. As the product had not yet been tested in this species, we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial program.

Simon Boudreault

Verified customer

Submitted May 30 2018

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