Key features and details
- Rabbit polyclonal to FLIP delta + gamma
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FLIP delta + gamma antibody
DescriptionRabbit polyclonal to FLIP delta + gamma
SpecificityThis antibody recognizes human and murine FLIP gamma and FLIP delta It does not recognise FLIP alpha and FLIP beta.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: HeLa, Jurkat , THP-1, A431, K562, and NIH3T3 cell lysates. ICC/IF: HeLa and Jurkat cells. ICC: HeLa and Jurkat cells.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
Primary antibody notesApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively (1,2). The human FLIPs were also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and usurpin (3-7). FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and –10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIP alpha, beta, gamma, and delta, respectively (8).
Light chain typeunknown
Our Abpromise guarantee covers the use of ab8423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 35 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
RelevanceFLIP (or CASP8 and FADD-like apoptosis regulator) is an apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
- CASP8 and FADD like apoptosis regulator antibody
- Caspase like apoptosis regulatory protein antibody
- Cellular FLICE like inhibitory protein antibody
ab8423 at 20μg/ml staining FLIP delta + gamma in Jurkat cells by ICC/IF (Immunocytochemistry/Immunofluorescence).
All lanes : Anti-FLIP delta + gamma antibody (ab8423) at 1/1000 dilution
Lane 1 : HeLa total cell lysate
Lane 2 : Jurkat total cell lysate
Lane 3 : THP1 total cell lysate
Lane 4 : A431 total cell lysate
Lane 5 : K562 total cell lysate
Lane 6 : NIH3T3 cells
Observed band size: 25,35 kDa why is the actual band size different from the predicted?
ab8423 at 20μg/ml staining FLIP delta + gamma in Jurkat cells by ICC (Immunocytochemistry).
ab8423 at 2µg/ml staining FLIP delta + gamma in Hela cells by Immunocytochemistry.
All lanes : Anti-FLIP delta + gamma antibody (ab8423) at 1/5000 dilution
Lanes 1-3 : TF-1 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/4000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 30,44,98 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
ab8423 has been referenced in 1 publication.
- Rushworth SA & MacEwan DJ HO-1 underlies resistance of AML cells to TNF-induced apoptosis. Blood 111:3793-801 (2008). PubMed: 18202225