Western blot abreview for Anti-FLIP delta + gamma antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (TF-1 cells)
Loading amount
30 µg
Specification
TF-1 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5%

Other product details

Dilution
1/5000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: blocking buffer (5% milk in tween +pbs)

Secondary antibody

Dilution
1/4000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 44, 30, 98 kDa
Positive control
jurkat cell lystae

Additional data

Additional Notes
Lanes 1-3 are TF-1 cell lysates (triplicate)
J = is jurkat cell lysate

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Submitted Jul 05 2007

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