Key features and details
- Rabbit polyclonal to FLJ20530
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FLJ20530 antibody
See all FLJ20530 primary antibodies
DescriptionRabbit polyclonal to FLJ20530
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
Synthetic peptide (Human) - the immunogen lies in the region between residues 200 and 250 of human FLJ20530/ KAONASHI1 using the numbering given in TrEMBL entry Q7Z314 (GeneID 55656).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
Our Abpromise guarantee covers the use of ab18050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 120 kDa.|
RelevanceFLJ20530 is a component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3' box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
- C8orf52 antibody
- Chromosome 8 open reading frame 52 antibody
- FLJ20530 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FLJ20530 at 1/1000 (1µg/ml). Detection: DAB.
Western blot using ab18050 at 0.2
µg/ml on HeLa nuclear lysate.
Detection: Chemiluminescence with an exposure time of 20 minutes. Western blot using ab18050 at 0.2µg/ml on HeLa nuclear lysate.
Detection: Chemiluminescence with an exposure time of 20 minutes.
ab18050 has not yet been referenced specifically in any publications.