Key features and details
- Mouse polyclonal to FLJ35220
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FLJ35220 antibody
DescriptionMouse polyclonal to FLJ35220
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Full length protein, corresponding to amino acids 1-264 of Human FLJ35220 (AAH64545.1)
- FLJ35220 transfected 293T cell lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab69400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
RelevanceThis gene encodes hypothetical protein FLJ35220
- hypothetical protein FLJ35220 antibody
- hypothetical protein LOC284131 antibody
- MGC74455 antibody
All lanes : Anti-FLJ35220 antibody (ab69400) at 1/500 dilution
Lane 1 : FLJ35220 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
ab69400 at 10µg/ml staining FLJ35220 in Hela cells by ICC/IF.
ab69400 has been referenced in 2 publications.
- Berges N et al. Complex alternative splicing of human Endonuclease V mRNA, but evidence for only a single protein isoform. PLoS One 14:e0225081 (2019). PubMed: 31703097
- Nawaz MS et al. Regulation of Human Endonuclease V Activity and Relocalization to Cytoplasmic Stress Granules. J Biol Chem 291:21786-21801 (2016). PubMed: 27573237