Key features and details
- Mouse monoclonal [Clone 10] to FLK1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Product nameAnti-FLK1 antibody [Clone 10]
DescriptionMouse monoclonal [Clone 10] to FLK1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Recombinant fragment corresponding to Saccharomyces cerevisiae FLK1 aa 1-200.
- Mav108 cells expressing recombinant FLK1.
This product was previously labelled as TUP1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
Clone numberClone 10
Our Abpromise guarantee covers the use of ab24313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa).|
RelevanceYeast TUP1 Ssn6p repressor complex represents a novel paradigm for transcriptional repression and for the role of chromatin in repression. TUP1 and SSN6 are involved in repression of several diverse families of genes in yeast, including cell type specific genes regulated by the alpha2 and a1/alpha2 repressors. Ssn6 Tupl interacts with class I histone deacetylases required for repression, and recruitment of yeast Tuplp Ssn6p repressor is associated with localised decreases in histone acetylation.
- FLK1 antibody
- Flocculation suppressor protein antibody
- Repressor AER2 antibody
All lanes : Anti-FLK1 antibody [Clone 10] (ab24313)
All lanes : Saccharomyces cerevisiae whole cell lysates
Lysates/proteins at 15 µg per lane.
All lanes : Sheep anti-Mouse polyclonal HRP conjugate at 100 %
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab24313 has been referenced in 1 publication.
- Mei B et al. The regulatory mechanism and biological significance of the Snail-miR590-VEGFR-NRP1 axis in the angiogenesis, growth and metastasis of gastric cancer. Cell Death Dis 11:241 (2020). PubMed: 32303680