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  1. Link

    flotillin-1-antibody-ab41927.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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Validated using a knockout cell line

Anti-Flotillin 1 antibody (ab41927)

  • Datasheet
  • SDS
Reviews (10)Q&A (5)References (67)

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Western blot - Anti-Flotillin 1 antibody (ab41927)
  • Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody (ab41927)
  • Western blot - Anti-Flotillin 1 antibody (ab41927)
  • Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody (ab41927)
  • Western blot - Anti-Flotillin 1 antibody (ab41927)

Key features and details

  • Rabbit polyclonal to Flotillin 1
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Flotillin 1 antibody [EPR6041] (ab133497)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Flotillin 1 antibody
    See all Flotillin 1 primary antibodies
  • Description

    Rabbit polyclonal to Flotillin 1
  • Host species

    Rabbit
  • Specificity

    This target has been reported to show both cytoplasmic and nuclear localisation (PMID: 15713644).
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human Flotillin 1 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab41926)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Immunizing Peptide (Blocking)

    • Human Flotillin 1 peptide (ab41926)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Flotillin 1 protein (ab153058)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab41927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
1/200. Fix cells with methanol
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with Human Flotillin 1 peptide (ab41926).
Notes
ICC/IF
1/200. Fix cells with methanol
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with Human Flotillin 1 peptide (ab41926).

Target

  • Function

    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
  • Sequence similarities

    Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • Cellular localization

    Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession O75955 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10211 Human
    • Entrez Gene: 14251 Mouse
    • Entrez Gene: 64665 Rat
    • Omim: 606998 Human
    • SwissProt: O75955 Human
    • SwissProt: O08917 Mouse
    • SwissProt: Q9Z1E1 Rat
    • Unigene: 179986 Human
    • Unigene: 2931 Mouse
    • Unigene: 9567 Rat
    see all
  • Alternative names

    • FLOT 1 antibody
    • FLOT1 antibody
    • FLOT1_HUMAN antibody
    • Flotillin-1 antibody
    • Flotillin1 antibody
    • Integral membrane component of caveolae antibody
    • Reggie 2 antibody
    see all

Images

  • Western blot - Anti-Flotillin 1 antibody (ab41927)
    Western blot - Anti-Flotillin 1 antibody (ab41927)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Flotillin 1 knockout HAP1 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab41927 observed at 47 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab41927 was shown to specifically react with Flotillin 1 in wild-type HAP1 cells as signal was lost in Flotillin 1 knockout cells. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab41927 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody (ab41927)
    Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody (ab41927)This image is part of an abreview submitted by Dr. Kirk McManus
    ab41927 (1/200) staining Flotillin 1 in HeLa cells (green). Cells were fixed with Methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

  • Western blot - Anti-Flotillin 1 antibody (ab41927)
    Western blot - Anti-Flotillin 1 antibody (ab41927)
    All lanes : Anti-Flotillin 1 antibody (ab41927) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody (ab41927)
    Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 1 antibody (ab41927)

    ICC/IF image of ab41927 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41927 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Flotillin 1 antibody (ab41927)
    Western blot - Anti-Flotillin 1 antibody (ab41927)
    All lanes : Anti-Flotillin 1 antibody (ab41927) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (67)

Publishing research using ab41927? Please let us know so that we can cite the reference in this datasheet.

ab41927 has been referenced in 67 publications.

  • Salvany S  et al. Microglial recruitment and mechanisms involved in the disruption of afferent synaptic terminals on spinal cord motor neurons after acute peripheral nerve injury. Glia 69:1216-1240 (2021). PubMed: 33386754
  • Lagoutte-Renosi J  et al. Influence of Antiplatelet Agents on the Lipid Composition of Platelet Plasma Membrane: A Lipidomics Approach with Ticagrelor and Its Active Metabolite. Int J Mol Sci 22:N/A (2021). PubMed: 33572690
  • Uysal-Onganer P  et al. Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures. Int J Mol Sci 22:N/A (2021). PubMed: 33573274
  • D'Alessio S  et al. Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer (Rangifer tarandus). Biology (Basel) 10:N/A (2021). PubMed: 33805829
  • Zhang Y  et al. Extracellular vesicle-encapsulated microRNA-23a from dorsal root ganglia neurons binds to A20 and promotes inflammatory macrophage polarization following peripheral nerve injury. Aging (Albany NY) 13:6752-6764 (2021). PubMed: 33621204
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Flotillin 1 antibody

Inconclusive
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (embryos)
Specification
embryos
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 23 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-Flotillin 1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Read More

DR. Kirk Mcmanus

Verified customer

Submitted Feb 18 2008

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