Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP446Y] to Flotillin 1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Product nameAnti-Flotillin 1 antibody [EP446Y] - BSA and Azide free
See all Flotillin 1 primary antibodies
DescriptionRabbit monoclonal [EP446Y] to Flotillin 1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Flotillin 1 aa 50-150. The exact sequence is proprietary.
Database link: O75955
Ab247493 is the carrier-free version of ab78178. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247493 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab247493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.|
FunctionMay act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Sequence similaritiesBelongs to the band 7/mec-2 family. Flotillin subfamily.
Cellular localizationCell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- FLOT 1 antibody
- FLOT1 antibody
- FLOT1_HUMAN antibody
All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : FLOT1 knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab78178).
ab78178 Anti-Flotillin 1 antibody [EP446Y] was shown to specifically react with Flotillin 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267276 (knockout cell lysate ab257109) was used. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab78178 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247493 has not yet been referenced specifically in any publications.