Product nameFlotillin 2 / ESA overexpression 293T lysate (whole cell)
See all Flotillin 2/ESA lysates
DescriptionFlotillin 2/ESA overexpression lysate
ab94260 is a 293T cell transfected lysate in which Human Flotillin 2 / ESA has been transiently over-expressed using a pCMV-Flotillin 2 / ESA plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundFunction: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. Tissue specificity: In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. Similarity: Belongs to the band 7/mec-2 family. Flotillin subfamily.
Our Abpromise guarantee covers the use of ab94260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94260 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Flotillin 2/ESA antibody (ab88498) at 1/500 dilution
Lane 1 :
Flotillin 2 / ESA overexpression 293T lysate (whole cell) (ab94260)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94260 has not yet been referenced specifically in any publications.