• Product name
  • Description
    Rabbit polyclonal to Flotillin 2
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within the C terminal amino acids 365-428 of Human Flotillin 2.

  • Positive control
    • HeLa S3 cell lysate, Lung CA (IHC)



Our Abpromise guarantee covers the use of ab96507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
ICC/IF 1/100 - 1/200.
IP Use at an assay dependent concentration.


  • Function
    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
  • Tissue specificity
    In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
  • Sequence similarities
    Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • Cellular localization
    Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae.
  • Information by UniProt
  • Database links
  • Alternative names
    • ECS-1 antibody
    • ECS1 antibody
    • Epidermal surface antigen 1 antibody
    • Epidermal surface antigen antibody
    • ESA antibody
    • ESA1 antibody
    • Flot2 antibody
    • FLOT2_HUMAN antibody
    • Flotillin 2 (epidermal surface antigen 1) antibody
    • Flotillin-2 antibody
    • M17S1 antibody
    • Membrane component chromosome 17 surface marker 1 antibody
    • Membrane component chromosome 17 surface marker 1 homolog antibody
    • membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) antibody
    • Membrane component, chromosome 17, surface marker 1 antibody
    • REG-1 antibody
    • Reggie-1 antibody
    • Reggie-2 antibody
    see all


  • Anti-Flotillin 2 antibody (ab96507) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg

    Predicted band size: 47 kDa

    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Lung CA, using ab96507 at 1:100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1:100 dilution. Image 2: Merged with DNA probe.
  • Flotillin 2 immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.

    Lane 1 : anti-Flotillin 2 IP (using non-Abcam antibody)
    Lane 2 : anti-Flotillin 2 IP (using ab96507)
    Lane 3 : Input (HeLa cell lysate)
    Lane 4 : Supernatant IP (using non-Abcam antibody)
    Lane 5 : Supernatant IP (using ab96507)

    Western blot detection : mouse anti-Flotillin 2 antibody + HRP donkey anti-mouse polyclonal secondary antibody

    See Abreview


This product has been referenced in:
  • Gong J  et al. Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance. J Cell Biol 214:35-44 (2016). Read more (PubMed: 27354374) »
  • Zhu Q  et al. Overexpression of acylglycerol kinase is associated with poorer prognosis and lymph node metastasis in nasopharyngeal carcinoma. Tumour Biol 37:3349-57 (2016). WB, IHC . Read more (PubMed: 26443540) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell lysate - whole cell (HeLa)
Total protein in input
750 µg
Immuno-precipitation step
Protein A

Prof. Ritva Tikkanen

Verified customer

Submitted Jan 11 2013

I am very pleased to hear you would like to test both ab41927 and ab96507 in immunoprecipitation. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for immunoprecipitation and in...

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