Anti-Flotillin 2/ESA antibody (ab113661)
Key features and details
- Goat polyclonal to Flotillin 2/ESA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Flotillin 2/ESA antibody
See all Flotillin 2/ESA primary antibodies -
Description
Goat polyclonal to Flotillin 2/ESA -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human Flotillin 2/ESA aa 415-428 (C terminal).
Sequence:C-SKIPLIKKATGVQV
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Positive control
- Human Kidney and Human Tonsil tissue. Human Brain (Cerebellum) lysate
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General notes
Previously labelled as Flotillin 2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab113661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 47 kDa. | |
| IHC-P | Use a concentration of 3.75 µg/ml. |
Target
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Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. -
Tissue specificity
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. -
Sequence similarities
Belongs to the band 7/mec-2 family. Flotillin subfamily. -
Cellular localization
Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 615679 Cow
- Entrez Gene: 2319 Human
- Omim: 131560 Human
- SwissProt: A6QLR4 Cow
- SwissProt: Q14254 Human
- Unigene: 514038 Human
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Alternative names
- ECS-1 antibody
- ECS1 antibody
- Epidermal surface antigen 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (ab113661)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human Kidney with ab113661 at 3.75ug/ml
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (ab113661)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human Tonsil with ab113661 at 3.75ug/ml -
Western blot - Anti-Flotillin 2/ESA antibody (ab113661)Anti-Flotillin 2/ESA antibody (ab113661) at 0.1 µg/ml + Human Brain (Cerebellum) lysate at 35 µg/ml
Developed using the ECL technique.
Predicted band size: 47 kDa
Primary incubation was 1 hour.
Protocols
Datasheets and documents
References (0)
ab113661 has not yet been referenced specifically in any publications.
