Anti-Flotillin 2/ESA antibody (ab113661)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Flotillin 2/ESA antibody
See all Flotillin 2/ESA primary antibodies -
Description
Goat polyclonal to Flotillin 2/ESA -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-P, ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human Flotillin 2/ESA aa 415-428 (C terminal).
Sequence:C-SKIPLIKKATGVQV
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Positive control
- Human Kidney and Human Tonsil tissue. Human Brain (Cerebellum) lysate
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General notes
Previously labelled as Flotillin 2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab113661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 47 kDa. | |
| IHC-P | Use a concentration of 3.75 µg/ml. | |
| ELISA | Use at an assay dependent concentration. |
Target
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Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. -
Tissue specificity
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. -
Sequence similarities
Belongs to the band 7/mec-2 family. Flotillin subfamily. -
Cellular localization
Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 615679 Cow
- Entrez Gene: 2319 Human
- Omim: 131560 Human
- SwissProt: A6QLR4 Cow
- SwissProt: Q14254 Human
- Unigene: 514038 Human
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Alternative names
- ECS-1 antibody
- ECS1 antibody
- Epidermal surface antigen 1 antibody
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Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (ab113661)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human Kidney with ab113661 at 3.75ug/ml -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (ab113661)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human Tonsil with ab113661 at 3.75ug/ml -
Western blot - Anti-Flotillin 2/ESA antibody (ab113661)Anti-Flotillin 2/ESA antibody (ab113661) at 0.1 µg/ml + Human Brain (Cerebellum) lysate at 35 µg/ml
Developed using the ECL technique.
Predicted band size: 47 kDa
Primary incubation was 1 hour.
Datasheets and documents
References
ab113661 has not yet been referenced specifically in any publications.
