Key features and details
- Goat polyclonal to Flotillin 2/ESA
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Flotillin 2/ESA antibody
See all Flotillin 2/ESA primary antibodies
DescriptionGoat polyclonal to Flotillin 2/ESA
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
- Human lung lysate.
Previously labelled as Flotillin 2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab20399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 µg/ml. Detects a band of approximately 48 kDa. Please note that this is significantly higher than the predicted size of 42kDa but agrees with the findings of Rajendran et al (see ref below). The 48kDa band was successfully blocked by incubation with the immunizing peptide. Use RIPA buffer.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Tissue specificityIn skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Sequence similaritiesBelongs to the band 7/mec-2 family. Flotillin subfamily.
Cellular localizationCell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae.
- Information by UniProt
- ECS-1 antibody
- ECS1 antibody
- Epidermal surface antigen 1 antibody
Anti-Flotillin 2/ESA antibody (ab20399) at 1 µg/ml + Human lung lysate at 0.3 µg/ml
Predicted band size: 42 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab20399 has been referenced in 1 publication.
- Lach A et al. Palmitoylation of MPP1 (membrane-palmitoylated protein 1)/p55 is crucial for lateral membrane organization in erythroid cells. J Biol Chem 287:18974-84 (2012). WB ; Human . PubMed: 22496366