• Product name

    Anti-Flotillin 2/ESA antibody
    See all Flotillin 2/ESA primary antibodies
  • Description

    Goat polyclonal to Flotillin 2/ESA
  • Host species

  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Flotillin 2/ESA aa 366-379 (C terminal).


  • Positive control

    • Human lung lysate.
  • General notes

    Previously labelled as Flotillin 2. 



Our Abpromise guarantee covers the use of ab20399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    Peptide ELISA: 1/32000.
    WB: Use at a concentration of 0.3 µg/ml. Detects a band of approximately 48 kDa. Please note that this is significantly higher than the predicted size of 42kDa but agrees with the findings of Rajendran et al (see ref below). The 48kDa band was successfully blocked by incubation with the immunizing peptide. Use RIPA buffer.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
    • Tissue specificity

      In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
    • Sequence similarities

      Belongs to the band 7/mec-2 family. Flotillin subfamily.
    • Cellular localization

      Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae.
    • Information by UniProt
    • Database links

    • Alternative names

      • ECS-1 antibody
      • ECS1 antibody
      • Epidermal surface antigen 1 antibody
      • Epidermal surface antigen antibody
      • ESA antibody
      • ESA1 antibody
      • Flot2 antibody
      • FLOT2_HUMAN antibody
      • Flotillin 2 (epidermal surface antigen 1) antibody
      • Flotillin-2 antibody
      • M17S1 antibody
      • Membrane component chromosome 17 surface marker 1 antibody
      • Membrane component chromosome 17 surface marker 1 homolog antibody
      • membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) antibody
      • Membrane component, chromosome 17, surface marker 1 antibody
      • REG-1 antibody
      • Reggie-1 antibody
      • Reggie-2 antibody
      see all


    • Anti-Flotillin 2/ESA antibody (ab20399) at 1 µg/ml + Human lung lysate at 0.3 µg/ml

      Predicted band size: 42 kDa
      Observed band size: 48 kDa
      why is the actual band size different from the predicted?

      Primary incubation was 1 hour. Detected by chemiluminescence.


    This product has been referenced in:

    • Lach A  et al. Palmitoylation of MPP1 (membrane-palmitoylated protein 1)/p55 is crucial for lateral membrane organization in erythroid cells. J Biol Chem 287:18974-84 (2012). WB ; Human . Read more (PubMed: 22496366) »
    See 1 Publication for this product

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