Product nameAnti-Flotillin 2/ESA antibody
See all Flotillin 2/ESA primary antibodies
DescriptionRabbit polyclonal to Flotillin 2/ESA
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Flotillin 2/ESA aa 365-428 (C terminal).
- HeLa S3 cell lysate, Lung CA (IHC)
Previously labelled as Flotillin 2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 2.68% PBS, 20% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 47 kDa.|
|IHC-P||1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.|
|ICC/IF||1/100 - 1/200.|
|IP||Use at an assay dependent concentration.|
FunctionMay act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Tissue specificityIn skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Sequence similaritiesBelongs to the band 7/mec-2 family. Flotillin subfamily.
Cellular localizationCell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae.
- Information by UniProt
- ECS-1 antibody
- ECS1 antibody
- Epidermal surface antigen 1 antibody
Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Lung CA, using ab96507 at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1:100 dilution. Image 2: Merged with DNA probe.
Flotillin 2/ESA immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.
Lane 1 : anti-Flotillin 2/ESA IP (using non-Abcam antibody)
Lane 2 : anti-Flotillin 2/ESA IP (using ab96507)
Lane 3 : Input (HeLa cell lysate)
Lane 4 : Supernatant IP (using non-Abcam antibody)
Lane 5 : Supernatant IP (using ab96507)
Western blot detection : mouse anti-Flotillin 2/ESA antibody + HRP donkey anti-mouse polyclonal secondary antibody
This product has been referenced in:
- Gong J et al. Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance. J Cell Biol 214:35-44 (2016). Read more (PubMed: 27354374) »
- Zhu Q et al. Overexpression of acylglycerol kinase is associated with poorer prognosis and lymph node metastasis in nasopharyngeal carcinoma. Tumour Biol 37:3349-57 (2016). WB, IHC . Read more (PubMed: 26443540) »