Overview

  • Product name
    Anti-Flotillin 2/ESA antibody
    See all Flotillin 2/ESA primary antibodies
  • Description
    Rabbit polyclonal to Flotillin 2/ESA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Flotillin 2/ESA aa 365-428 (C terminal).

  • Positive control
    • HeLa S3 cell lysate, Lung CA (IHC)
  • General notes

    Previously labelled as Flotillin 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab96507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
ICC/IF 1/100 - 1/200.
IP Use at an assay dependent concentration.

Target

  • Function
    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
  • Tissue specificity
    In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
  • Sequence similarities
    Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • Cellular localization
    Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae.
  • Information by UniProt
  • Database links
  • Alternative names
    • ECS-1 antibody
    • ECS1 antibody
    • Epidermal surface antigen 1 antibody
    • Epidermal surface antigen antibody
    • ESA antibody
    • ESA1 antibody
    • Flot2 antibody
    • FLOT2_HUMAN antibody
    • Flotillin 2 (epidermal surface antigen 1) antibody
    • Flotillin-2 antibody
    • M17S1 antibody
    • Membrane component chromosome 17 surface marker 1 antibody
    • Membrane component chromosome 17 surface marker 1 homolog antibody
    • membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) antibody
    • Membrane component, chromosome 17, surface marker 1 antibody
    • REG-1 antibody
    • Reggie-1 antibody
    • Reggie-2 antibody
    see all

Images

  • Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Lung CA, using ab96507 at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1:100 dilution. Image 2: Merged with DNA probe.
  • Flotillin 2/ESA immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.

    Lane 1 : anti-Flotillin 2/ESA IP (using non-Abcam antibody)
    Lane 2 : anti-Flotillin 2/ESA IP (using ab96507)
    Lane 3 : Input (HeLa cell lysate)
    Lane 4 : Supernatant IP (using non-Abcam antibody)
    Lane 5 : Supernatant IP (using ab96507)

    Western blot detection : mouse anti-Flotillin 2/ESA antibody + HRP donkey anti-mouse polyclonal secondary antibody

    See Abreview

References

This product has been referenced in:
  • Gong J  et al. Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance. J Cell Biol 214:35-44 (2016). Read more (PubMed: 27354374) »
  • Zhu Q  et al. Overexpression of acylglycerol kinase is associated with poorer prognosis and lymph node metastasis in nasopharyngeal carcinoma. Tumour Biol 37:3349-57 (2016). WB, IHC . Read more (PubMed: 26443540) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

I am very pleased to hear you would like to test both ab41927 and ab96507 in immunoprecipitation. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for immunoprecipitation and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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