Recombinant
RabMAb

Recombinant Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)

Overview

  • Product name

    Anti-Flotillin 2/ESA antibody [EPR14128(B)]
    See all Flotillin 2/ESA primary antibodies
  • Description

    Rabbit monoclonal [EPR14128(B)] to Flotillin 2/ESA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Flotillin 2/ESA aa 300-400. The exact sequence is proprietary.
    Database link: Q14254

  • Positive control

    • 293, Hela, A375 and A549 cell line
  • General notes

     

     

    Previously labelled as Flotillin 2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 47 kDa).
IP 1/40.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
  • Tissue specificity

    In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
  • Sequence similarities

    Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • Cellular localization

    Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae.
  • Information by UniProt
  • Database links

  • Alternative names

    • ECS-1 antibody
    • ECS1 antibody
    • Epidermal surface antigen 1 antibody
    • Epidermal surface antigen antibody
    • ESA antibody
    • ESA1 antibody
    • Flot2 antibody
    • FLOT2_HUMAN antibody
    • Flotillin 2 (epidermal surface antigen 1) antibody
    • Flotillin-2 antibody
    • M17S1 antibody
    • Membrane component chromosome 17 surface marker 1 antibody
    • Membrane component chromosome 17 surface marker 1 homolog antibody
    • membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) antibody
    • Membrane component, chromosome 17, surface marker 1 antibody
    • REG-1 antibody
    • Reggie-1 antibody
    • Reggie-2 antibody
    see all

Images

  • All lanes : Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988) at 1/4000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : A375 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 47 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Immunoprecipitation of Hela cell lysate labeling Flotillin 2/ESA using ab181988 at a 1/40 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.

References

ab181988 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - other (Liver)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
65 µg
Specification
Liver
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 18 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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