Recombinant Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)
![Western blot - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)](https://www.abcam.com/ps/products/181/ab181988/Images/ab181988-395495-anti-flotillin-2esa-antibody-epr14128-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14128(B)] to Flotillin 2/ESA
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Flotillin 2/ESA antibody [EPR14128(B)]
See all Flotillin 2/ESA primary antibodies -
Description
Rabbit monoclonal [EPR14128(B)] to Flotillin 2/ESA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293, Hela, A375 and A549 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14128(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 47 kDa).
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| IP |
1/40.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 47 kDa). |
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IP
1/40. |
Target
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Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. -
Tissue specificity
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. -
Sequence similarities
Belongs to the band 7/mec-2 family. Flotillin subfamily. -
Cellular localization
Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 2319 Human
- Entrez Gene: 14252 Mouse
- Entrez Gene: 83764 Rat
- Omim: 131560 Human
- SwissProt: Q14254 Human
- SwissProt: Q60634 Mouse
- SwissProt: Q9Z2S9 Rat
- Unigene: 514038 Human
see all -
Alternative names
- ECS-1 antibody
- ECS1 antibody
- Epidermal surface antigen 1 antibody
see all
Images
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Western blot - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)All lanes : Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : FLOT2 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaLanes 1-4: Merged signal (red and green). Green - ab181988 observed at 47 kDa. Red - loading control ab8245 observed at 36 kDa.
ab181988 Anti-Flotillin 2/ESA antibody [EPR14128(B)] was shown to specifically react with Flotillin 2/ESA in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266847 (knockout cell lysate ab257442) was used. Wild-type and Flotillin 2/ESA knockout samples were subjected to SDS-PAGE. ab181988 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)](https://www.abcam.com/ps/products/181/ab181988/Images/ab181988-212547-anti-flotillin-2-esa-antibody-epr14128b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)Immunoprecipitation of Hela cell lysate labeling Flotillin 2/ESA using ab181988 at a 1/40 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)](https://www.abcam.com/ps/products/181/ab181988/Images/ab181988-212546-anti-flotillin-2-esa-antibody-epr14128b-western-blot.jpg)
Western blot - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)All lanes : Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988) at 1/4000 dilution
Lane 1 : 293 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : A375 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDaBlocking buffer and concentration: 5% NFDM/TBST
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![Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)](https://www.abcam.com/ps/products/181/ab181988/Images/ab181988-4-benefits-of-recombinant-antibodies.png)
Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab181988 has been referenced in 1 publication.
- Chen D et al. Adenomyosis-derived extracellular vesicles endow endometrial epithelial cells with an invasive phenotype through epithelial-mesenchymal transition. Genes Dis 7:636-648 (2020). PubMed: 33335963
