Anti-FLR antibody (ab231326)
Key features and details
- Rabbit polyclonal to FLR
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FLR antibody
See all FLR primary antibodies -
Description
Rabbit polyclonal to FLR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human FLR aa 2-206. Expressed in E.coli. N-terminal tags.
Sequence:AVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVV VGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAH GVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMP PHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYP SHQYQ
Database link: P30043 -
Positive control
- WB: Human liver and lung tissue; Rat liver tissue and HeLa cell lysate. IHC-P: Human glioma, cerebrum, liver and kidney tissue; Human lung cancer tissue. ICC: HeLa cell lysate.
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General notes
This product was previously labelled as BLVRB
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab231326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 - 20 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. | |
| ICC/IF | Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin. -
Tissue specificity
Predominantly expressed in liver and erythrocytes. At lower levels in heart, lung, adrenal gland and cerebrum. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 281650 Cow
- Entrez Gene: 645 Human
- Entrez Gene: 233016 Mouse
- Entrez Gene: 292737 Rat
- Omim: 600941 Human
- SwissProt: P52556 Cow
- SwissProt: P30043 Human
- SwissProt: Q923D2 Mouse
see all -
Alternative names
- Biliverdin IX beta reductase antibody
- biliverdin reductase B (flavin reductase (NADPH)) antibody
- Biliverdin reductase B antibody
see all
Images
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Western blot - Anti-FLR antibody (ab231326)All lanes : Anti-FLR antibody (ab231326) at 2 µg/ml
Lane 1 : Wild type HeLa cells
Lane 2 : FLR knockout HeLa cells -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)DAB stained formalin-fixed paraffin-embedded human kidney tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
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Western blot - Anti-FLR antibody (ab231326)All lanes : Anti-FLR antibody (ab231326) at 1 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-FLR antibody (ab231326)Paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for FLR (green) using ab231326 at 20 µg/ml in ICC/IF. FITC staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)DAB stained formalin-fixed paraffin-embedded human lung cancer tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)DAB stained formalin-fixed paraffin-embedded human liver tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)DAB stained formalin-fixed paraffin-embedded human cerebrum tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)DAB stained formalin-fixed paraffin-embedded human glioma tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
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Western blot - Anti-FLR antibody (ab231326)Anti-FLR antibody (ab231326) at 2 µg/ml + Recombinant human FLR protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab231326 has not yet been referenced specifically in any publications.
