Anti-FLR antibody (ab231326)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-FLR antibody
See all FLR primary antibodies -
Description
Rabbit polyclonal to FLR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow
-
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human FLR aa 2-206. Expressed in E.coli. N-terminal tags.
Sequence:AVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVV VGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAH GVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMP PHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYP SHQYQ
Database link: P30043 -
Positive control
- WB: Human liver and lung tissue; Rat liver tissue and HeLa cell lysate. IHC-P: Human glioma, cerebrum, liver and kidney tissue; Human lung cancer tissue. ICC: HeLa cell lysate.
-
General notes
This product was previously labelled as BLVRB
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab231326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 - 20 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. | |
| ICC/IF | Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin. -
Tissue specificity
Predominantly expressed in liver and erythrocytes. At lower levels in heart, lung, adrenal gland and cerebrum. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 281650 Cow
- Entrez Gene: 645 Human
- Entrez Gene: 233016 Mouse
- Entrez Gene: 292737 Rat
- Omim: 600941 Human
- SwissProt: P52556 Cow
- SwissProt: P30043 Human
- SwissProt: Q923D2 Mouse
see all -
Alternative names
- Biliverdin IX beta reductase antibody
- biliverdin reductase B (flavin reductase (NADPH)) antibody
- Biliverdin reductase B antibody
see all
Images
-
Western blot - Anti-FLR antibody (ab231326)All lanes : Anti-FLR antibody (ab231326) at 2 µg/ml
Lane 1 : Wild type HeLa cells
Lane 2 : FLR knockout HeLa cells -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)DAB stained formalin-fixed paraffin-embedded human kidney tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
-
Western blot - Anti-FLR antibody (ab231326)All lanes : Anti-FLR antibody (ab231326) at 1 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-FLR antibody (ab231326)Paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for FLR (green) using ab231326 at 20 µg/ml in ICC/IF. FITC staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)DAB stained formalin-fixed paraffin-embedded human lung cancer tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)DAB stained formalin-fixed paraffin-embedded human liver tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)DAB stained formalin-fixed paraffin-embedded human cerebrum tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)DAB stained formalin-fixed paraffin-embedded human glioma tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
-
Western blot - Anti-FLR antibody (ab231326)Anti-FLR antibody (ab231326) at 2 µg/ml + Recombinant human FLR protein
Datasheets and documents
References
ab231326 has not yet been referenced specifically in any publications.
