Key features and details
- Rabbit polyclonal to FLR
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-FLR antibody
See all FLR primary antibodies
DescriptionRabbit polyclonal to FLR
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow
Recombinant full length protein (His-T7-tag) corresponding to Human FLR aa 2-206. Expressed in E.coli. N-terminal tags.
AVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVV VGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAH GVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMP PHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYP SHQYQ
Database link: P30043
- WB: Human liver and lung tissue; Rat liver tissue and HeLa cell lysate. IHC-P: Human glioma, cerebrum, liver and kidney tissue; Human lung cancer tissue. ICC: HeLa cell lysate.
This product was previously labelled as BLVRB
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFollowed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionBroad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.
Tissue specificityPredominantly expressed in liver and erythrocytes. At lower levels in heart, lung, adrenal gland and cerebrum.
- Information by UniProt
- Biliverdin IX beta reductase antibody
- biliverdin reductase B (flavin reductase (NADPH)) antibody
- Biliverdin reductase B antibody
All lanes : Anti-FLR antibody (ab231326) at 2 µg/ml
Lane 1 : Wild type HeLa cells
Lane 2 : FLR knockout HeLa cells
DAB stained formalin-fixed paraffin-embedded human kidney tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
All lanes : Anti-FLR antibody (ab231326) at 1 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for FLR (green) using ab231326 at 20 µg/ml in ICC/IF. FITC staining.
DAB stained formalin-fixed paraffin-embedded human lung cancer tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
DAB stained formalin-fixed paraffin-embedded human liver tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
DAB stained formalin-fixed paraffin-embedded human cerebrum tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
DAB stained formalin-fixed paraffin-embedded human glioma tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.
Anti-FLR antibody (ab231326) at 2 µg/ml + Recombinant human FLR protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231326 has not yet been referenced specifically in any publications.