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    flr-antibody-ab231326.pdf

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Anti-FLR antibody (ab231326)

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Western blot - Anti-FLR antibody (ab231326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)
  • Western blot - Anti-FLR antibody (ab231326)
  • Immunocytochemistry/ Immunofluorescence - Anti-FLR antibody (ab231326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)
  • Western blot - Anti-FLR antibody (ab231326)

Key features and details

  • Rabbit polyclonal to FLR
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human FLR protein (ab79183)

View more associated products

Overview

  • Product name

    Anti-FLR antibody
    See all FLR primary antibodies
  • Description

    Rabbit polyclonal to FLR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant full length protein (His-T7-tag) corresponding to Human FLR aa 2-206. Expressed in E.coli. N-terminal tags.
    Sequence:

    AVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVV VGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAH GVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMP PHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYP SHQYQ


    Database link: P30043
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human liver and lung tissue; Rat liver tissue and HeLa cell lysate. IHC-P: Human glioma, cerebrum, liver and kidney tissue; Human lung cancer tissue. ICC: HeLa cell lysate.
  • General notes

     This product was previously labelled as BLVRB

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human FLR protein (ab79183)

Applications

Our Abpromise guarantee covers the use of ab231326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml.
ICC/IF Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.
  • Tissue specificity

    Predominantly expressed in liver and erythrocytes. At lower levels in heart, lung, adrenal gland and cerebrum.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P30043 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281650 Cow
    • Entrez Gene: 645 Human
    • Entrez Gene: 233016 Mouse
    • Entrez Gene: 292737 Rat
    • Omim: 600941 Human
    • SwissProt: P52556 Cow
    • SwissProt: P30043 Human
    • SwissProt: Q923D2 Mouse
    • Unigene: 515785 Human
    • Unigene: 24021 Mouse
    • Unigene: 161812 Rat
    see all
  • Alternative names

    • Biliverdin IX beta reductase antibody
    • biliverdin reductase B (flavin reductase (NADPH)) antibody
    • Biliverdin reductase B antibody
    • Biliverdin-IX beta-reductase antibody
    • BLVRB antibody
    • BLVRB_HUMAN antibody
    • BVR B antibody
    • BVR-B antibody
    • BVRB antibody
    • epididymis secretory protein Li 10 antibody
    • Flavin reductase (NADPH) antibody
    • Flavin reductase antibody
    • FLR antibody
    • FR antibody
    • GHBP antibody
    • Green heme binding protein antibody
    • Green heme-binding protein antibody
    • HEL-S-10 antibody
    • Methemoglobin reductase antibody
    • MGC117413 antibody
    • NADPH dependent diaphorase antibody
    • NADPH flavin reductase antibody
    • NADPH-dependent diaphorase antibody
    • NADPH-flavin reductase antibody
    • SDR43U1 antibody
    • short chain dehydrogenase/reductase family 43U, member 1 antibody
    see all

Images

  • Western blot - Anti-FLR antibody (ab231326)
    Western blot - Anti-FLR antibody (ab231326)
    All lanes : Anti-FLR antibody (ab231326) at 2 µg/ml

    Lane 1 : Wild type HeLa cells
    Lane 2 : FLR knockout HeLa cells
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)

    DAB stained formalin-fixed paraffin-embedded human kidney tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.

  • Western blot - Anti-FLR antibody (ab231326)
    Western blot - Anti-FLR antibody (ab231326)
    All lanes : Anti-FLR antibody (ab231326) at 1 µg/ml

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human lung tissue lysate
    Lane 3 : Rat liver tissue lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Secondary
    All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
  • Immunocytochemistry/ Immunofluorescence - Anti-FLR antibody (ab231326)
    Immunocytochemistry/ Immunofluorescence - Anti-FLR antibody (ab231326)

    Paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for FLR (green) using ab231326 at 20 µg/ml in ICC/IF. FITC staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)

    DAB stained formalin-fixed paraffin-embedded human lung cancer tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)

    DAB stained formalin-fixed paraffin-embedded human liver tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)

    DAB stained formalin-fixed paraffin-embedded human cerebrum tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLR antibody (ab231326)

    DAB stained formalin-fixed paraffin-embedded human glioma tissue stained for FLR using ab231326 at 20 µg/ml in immunohistochemical analysis.

  • Western blot - Anti-FLR antibody (ab231326)
    Western blot - Anti-FLR antibody (ab231326)
    Anti-FLR antibody (ab231326) at 2 µg/ml + Recombinant human FLR protein

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab231326? Please let us know so that we can cite the reference in this datasheet.

    ab231326 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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