Overview

  • Product name

  • Description

    Rabbit polyclonal to FLRT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 63 and 253 of Human FLRT1 (NP_037412)

  • Positive control

    • 293T; A431; H1299; HeLaS3; HepG2; MOLT4 or Raji lysates OVCA (IHC-P)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P 1/100 - 1/250.

Target

  • Function

    Plays a role in fibroblast growth factor-mediated signaling cascades that lead to the activation of MAP kinases. Promotes neurite outgrowth via FGFR1-mediated activation of downstream MAP kinases. Promotes an increase both in neurite number and in neurite length. May play a role in cell-cell adhesion and cell guidance via its interaction with ADGRL1/LPHN1 and ADGRL3.
  • Tissue specificity

    Expressed in kidney and brain.
  • Sequence similarities

    Contains 1 fibronectin type-III domain.
    Contains 10 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications

    N-glycosylated.
    Phosphorylated in response to FGFR1 signaling, but is not a direct substrate of FGFR1 or SRC. A mutant where the Tyr phosphorylation sites have been replaced by Phe displays constitutive FGFR1-dependent activation of downstream MAP kinases.
    Proteolytic cleavage in the juxtamembrane region gives rise to a soluble ectodomain.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle membrane. Cytoplasm, perinuclear region. Cell junction, focal adhesion. Secreted. Cell projection. Cell junction. In addition to its location at the cell membrane, colocalizes with FGFR1 in punctate perinuclear cytoplasmic vesicles. Detected along neurites and at contacts between neurite termini and other cells. Proteolytic cleavage gives rise to a shedded ectodomain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fibronectin-like domain containing leucine rich transmembrane protein 1 antibody
    • Fibronectin-like domain-containing leucine-rich transmembrane protein 1 antibody
    • FLRT1 antibody
    • FLRT1_HUMAN antibody
    • Leucine rich repeat transmembrane protein FLRT1 antibody
    • Leucine-rich repeat transmembrane protein FLRT1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded OVCA, using ab97825 at 1:100 dilution.
  • All lanes : Anti-FLRT1 antibody (ab97825) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLaS3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 74 kDa



    7.5% SDS PAGE

References

ab97825 has not yet been referenced specifically in any publications.

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