Overview

  • Product name

    Anti-FLRT2 antibody
  • Description

    Rabbit polyclonal to FLRT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 58-256 of Human FLRT2 (UniProt: O43155).

  • Positive control

    • A431 whole cell lysate; Human Mahlarvu xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    May have a function in cell adhesion and/or receptor signaling.
  • Cellular localization

    Membrane; Single-pass type I membrane protein.
  • Database links

  • Alternative names

    • Fibronectin leucine rich transmembrane protein 2 antibody
    • Fibronectin-like domain-containing leucine-rich transmembrane protein 2 antibody
    • KIAA0405 antibody
    • Leucine rich repeat transmembrane protein antibody
    • Leucine rich repeat transmembrane protein FLRT2 antibody
    see all

Images

  • Anti-FLRT2 antibody (ab154023) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 74 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Mahlarvu xenograft tissue labeling FLRT2 with ab154023 at 1/500 dilution.

References

ab154023 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
10% FBS in DMEM with high glucose, L-glutamine and sodium pyruvate + 0.05% triton X100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Sample
Mouse Tissue sections (Mouse Retina)
Specification
Mouse Retina
Permeabilization
No
Fixative
Paraformaldehyde

Steven Perry

Verified customer

Submitted Nov 22 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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