Product nameAnti-Flt3 / CD135 antibody [EPR22232-403] - BSA and Azide free
See all Flt3 / CD135 primary antibodies
DescriptionRabbit monoclonal [EPR22232-403] to Flt3 / CD135 - BSA and Azide free
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB
Species reactivityReacts with: Human
Recombinant fragment within Human Flt3/ CD135 aa 1-550. The exact sequence is proprietary.
Database link: P36888
- Flow: THP-1 cells.
Ab245985 is the carrier-free version of ab245840. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab245985 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab245985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
FunctionReceptor for the FL cytokine. Has a tyrosine-protein kinase activity.
Tissue specificityBone marrow cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 protein kinase domain.
- Information by UniProt
- CD 135 antibody
- CD135 antibody
- CD135 antigen antibody
Flow cytometric analysis of 4% paraformaldehyde-fixed U-937 (human histiocytic lymphoma monocyte)(Left) and THP-1 (human monocytic leukemia cell lin)(Right) cells labeling Flt3 / CD135 with ab245840 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Dylight ® 488, ab98462) was used as the secondary antibody at 1/2000 dilution.
Gated on viable cells.
Negative control: U-937. (PMID: 12406902).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245840).
ab245985 has not yet been referenced specifically in any publications.