Recombinant
RabMAb

Recombinant Anti-Flt3 / CD135 antibody [EPR22232-403] - BSA and Azide free (ab245985)

Overview

  • Product name

    Anti-Flt3 / CD135 antibody [EPR22232-403] - BSA and Azide free
    See all Flt3 / CD135 primary antibodies
  • Description

    Rabbit monoclonal [EPR22232-403] to Flt3 / CD135 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Flt3/ CD135 aa 1-550. The exact sequence is proprietary.
    Database link: P36888

  • Positive control

    • Flow: THP-1 cells.
  • General notes

    Ab245985 is the carrier-free version of ab245840. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab245985 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab245985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P,IP or WB.
  • Target

    • Function

      Receptor for the FL cytokine. Has a tyrosine-protein kinase activity.
    • Tissue specificity

      Bone marrow cells.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 protein kinase domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 135 antibody
      • CD135 antibody
      • CD135 antigen antibody
      • Fetal liver kinase 2 antibody
      • FL cytokine receptor antibody
      • Flk 2 antibody
      • Flk2 antibody
      • Flt 3 antibody
      • FLT-3 antibody
      • Flt3 antibody
      • FLT3_HUMAN antibody
      • FMS like tyrosine kinase 3 antibody
      • Fms related tyrosine kinase 3 antibody
      • Fms-like tyrosine kinase 3 antibody
      • Growth factor receptor tyrosine kinase type III antibody
      • Ly-72 antibody
      • OTTHUMP0000004234 antibody
      • Receptor type tyrosine protein kinase FLT3 antibody
      • Stem cell tyrosine kinase 1 antibody
      • Stk 1 antibody
      • STK-1 antibody
      • Stk1 antibody
      • Tyrosine protein kinase receptor FLT3 antibody
      • Tyrosine-protein kinase receptor FLT3 antibody
      see all

    Images

    •  

      Flow cytometric analysis of 4% paraformaldehyde-fixed U-937 (human histiocytic lymphoma monocyte)(Left) and THP-1 (human monocytic leukemia cell lin)(Right) cells labeling Flt3 / CD135 with ab245840 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Dylight ® 488, ab98462) was used as the secondary antibody at 1/2000 dilution.

      Gated on viable cells.

      Negative control: U-937. (PMID: 12406902). 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245840).

    References

    ab245985 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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