Recombinant Anti-Flt3 / CD135 (phospho Y589) antibody [EPR2311(2)] - BSA and Azide free (ab249687)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2311(2)] to Flt3 / CD135 (phospho Y589) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Flt3 / CD135 (phospho Y589) antibody [EPR2311(2)] - BSA and Azide free
See all Flt3 / CD135 primary antibodies -
Description
Rabbit monoclonal [EPR2311(2)] to Flt3 / CD135 (phospho Y589) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Flt3/ CD135 aa 550-650 (phospho Y589) (Cysteine residue). The exact sequence is proprietary.
Database link: P36888 -
General notes
ab249687 is the carrier-free version of ab171975.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2311(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 130-160 kDa (predicted molecular weight: 112 kDa).
|
Notes |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 130-160 kDa (predicted molecular weight: 112 kDa). |
Target
-
Function
Receptor for the FL cytokine. Has a tyrosine-protein kinase activity. -
Tissue specificity
Bone marrow cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2322 Human
- Omim: 136351 Human
- SwissProt: P36888 Human
- Unigene: 507590 Human
-
Alternative names
- CD 135 antibody
- CD135 antibody
- CD135 antigen antibody
see all
Images
-
All lanes : Anti-Flt3 / CD135 (phospho Y589) antibody [EPR2311(2)] (ab171975) at 1/1000 dilution
Lane 1 : A431 cell lysate - untreated
Lane 2 : A431 cell lysate - treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDaThis data was developed using ab171975, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab249687 has not yet been referenced specifically in any publications.