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Immunology Adaptive Immunity B Cells CD
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Anti-Flt3 / CD135 antibody [MM0294-9G23] (ab89554)

  • Datasheet
  • SDS
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Western blot - Anti-Flt3 / CD135 antibody [MM0294-9G23] (ab89554)

    Key features and details

    • Mouse monoclonal [MM0294-9G23] to Flt3 / CD135
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-Flt3 / CD135 antibody [MM0294-9G23]
      See all Flt3 / CD135 primary antibodies
    • Description

      Mouse monoclonal [MM0294-9G23] to Flt3 / CD135
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant full length Human Flt3/ CD135 protein.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
    • Clonality

      Monoclonal
    • Clone number

      MM0294-9G23
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Receptor Tyrosine Kinases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant human Flt3 / CD135 protein (Fc Chimera Active) (ab83998)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab89554 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 113 kDa.
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 113 kDa.

    Target

    • Function

      Receptor for the FL cytokine. Has a tyrosine-protein kinase activity.
    • Tissue specificity

      Bone marrow cells.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 protein kinase domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P36888 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2322 Human
      • Omim: 136351 Human
      • SwissProt: P36888 Human
      • Unigene: 507590 Human
      • Alternative names

        • CD 135 antibody
        • CD135 antibody
        • CD135 antigen antibody
        • Fetal liver kinase 2 antibody
        • FL cytokine receptor antibody
        • Flk 2 antibody
        • Flk2 antibody
        • Flt 3 antibody
        • FLT-3 antibody
        • Flt3 antibody
        • FLT3_HUMAN antibody
        • FMS like tyrosine kinase 3 antibody
        • Fms related tyrosine kinase 3 antibody
        • Fms-like tyrosine kinase 3 antibody
        • Growth factor receptor tyrosine kinase type III antibody
        • Ly-72 antibody
        • OTTHUMP0000004234 antibody
        • Receptor type tyrosine protein kinase FLT3 antibody
        • Stem cell tyrosine kinase 1 antibody
        • Stk 1 antibody
        • STK-1 antibody
        • Stk1 antibody
        • Tyrosine protein kinase receptor FLT3 antibody
        • Tyrosine-protein kinase receptor FLT3 antibody
        see all

      Images

      • Western blot - Anti-Flt3 / CD135 antibody [MM0294-9G23] (ab89554)
        Western blot - Anti-Flt3 / CD135 antibody [MM0294-9G23] (ab89554)
        Anti-Flt3 / CD135 antibody [MM0294-9G23] (ab89554) at 1/500 dilution + Human placenta tissue lysate

        Predicted band size: 113 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab89554? Please let us know so that we can cite the reference in this datasheet.

      ab89554 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Flow Cytometry abreview for Anti-Flt3 / CD135 antibody [MM0294-9G23]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (cell line THP-1)
      Permeabilization
      No
      Gating Strategy
      Cy-3 positive cells
      Specification
      cell line THP-1
      Preparation
      Cell harvesting/tissue preparation method: cell culture
      Sample buffer: preparation in PBS
      Read More

      Dr. J Mueller

      Verified customer

      Submitted Jun 21 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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