Anti-Flt3 ligand/Flt3L antibody (Biotin) (ab84261)
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Overview
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Product name
Anti-Flt3 ligand/Flt3L antibody (Biotin)
See all Flt3 ligand/Flt3L primary antibodies -
Description
Rabbit polyclonal to Flt3 ligand/Flt3L (Biotin) -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Flt3 ligand/Flt3L.
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General notes
Previously labelled as Flt3 ligand.
Properties
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Form
Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab84261 is sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab84261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | ||
| WB |
Application notes
ELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand.
sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human Flt3 ligand is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions. Predicted molecular weight: 26 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human Flt3 ligand is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions. Predicted molecular weight: 26 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2323 Human
- Omim: 600007 Human
- SwissProt: P49771 Human
- Unigene: 428 Human
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Alternative names
- FL antibody
- Flt 3 ligand antibody
- Flt 3L antibody
see all
Protocols
Datasheets and documents
References
ab84261 has not yet been referenced specifically in any publications.
