Anti-Flt3 ligand/Flt3L antibody (Biotin) (ab84261)
Key features and details
- Rabbit polyclonal to Flt3 ligand/Flt3L (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Anti-Flt3 ligand/Flt3L antibody (Biotin)
See all Flt3 ligand/Flt3L primary antibodies -
Description
Rabbit polyclonal to Flt3 ligand/Flt3L (Biotin) -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Flt3 ligand/Flt3L.
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General notes
Previously labelled as Flt3 ligand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab84261 is sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab84261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand. |
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| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 26 kDa. The detection limit for recombinant human Flt3 ligand is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions. |
Target
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Function
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2323 Human
- Omim: 600007 Human
- SwissProt: P49771 Human
- Unigene: 428 Human
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Alternative names
- FL antibody
- Flt 3 ligand antibody
- Flt 3L antibody
see all
Images
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Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (Biotin) (ab84261)To detect human Flt3 ligand/Flt3L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84261 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Flt3 ligand/Flt3L (ab9688) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Flt3 ligand/Flt3L.
Datasheets and documents
References (0)
ab84261 has not yet been referenced specifically in any publications.
