Key features and details
- Rabbit polyclonal to Flt3 ligand/Flt3L (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Flt3 ligand/Flt3L antibody (Biotin)
See all Flt3 ligand/Flt3L primary antibodies
DescriptionRabbit polyclonal to Flt3 ligand/Flt3L (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Flt3 ligand/Flt3L.
Previously labelled as Flt3 ligand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Purification notesab84261 is sterile filtered.
Our Abpromise guarantee covers the use of ab84261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 26 kDa.
The detection limit for recombinant human Flt3 ligand is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions.
FunctionStimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- FL antibody
- Flt 3 ligand antibody
- Flt 3L antibody
To detect human Flt3 ligand/Flt3L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84261 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Flt3 ligand/Flt3L (ab9688) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Flt3 ligand/Flt3L.
ab84261 has not yet been referenced specifically in any publications.