Product nameAnti-Flt3 ligand/Flt3L antibody [EP1140Y]
See all Flt3 ligand/Flt3L primary antibodies
DescriptionRabbit monoclonal [EP1140Y] to Flt3 ligand/Flt3L
The antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand/Flt3L. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.
Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human, Rhesus monkey
Synthetic peptide corresponding to Human Flt3 ligand/Flt3L aa 150 to the C-terminus.
Database link: P49771
- IHC: Human liver. WB: recombinant protein ICC/IF: MCF7 FC: Hela
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Flt3 ligand.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab52648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/100000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/180. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionStimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- FL antibody
- Flt 3 ligand antibody
- Flt 3L antibody
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Flt3 ligand/Flt3L with Purified ab52648 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648) at 1/100000 dilution (purified) + Human Flt3 ligand/Flt3L (aa 1 to 235) recombinant protein, 15ng
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Flt3 ligand/Flt3L with ab52648 at 1:20 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) /(Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1:2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Flt3 ligand with purified ab52648 at 1:180 dilution (2.9 μg/ml). ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Yuan X et al. Mesenchymal stem cell therapy induces FLT3L and CD1c+ dendritic cells in systemic lupus erythematosus patients. Nat Commun 10:2498 (2019). Read more (PubMed: 31175312) »
- Traer E et al. FGF2 from Marrow Microenvironment Promotes Resistance to FLT3 Inhibitors in Acute Myeloid Leukemia. Cancer Res 76:6471-6482 (2016). Read more (PubMed: 27671675) »