Overview

  • Product name
    Anti-Flt3 ligand/Flt3L antibody [EP1140Y]
    See all Flt3 ligand/Flt3L primary antibodies
  • Description
    Rabbit monoclonal [EP1140Y] to Flt3 ligand/Flt3L
  • Host species
    Rabbit
  • Specificity

    The antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand/Flt3L. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.

  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Flt3 ligand/Flt3L aa 150-250.

  • Positive control
    • IHC: Human tonsil tissue; human liver. WB: recombinant protein ICC/IF: MCF7 FC: Hela
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as Flt3 ligand.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EP1140Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/100000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/10.

IHC-P 1/180. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100.

Target

  • Function
    Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FL antibody
    • Flt 3 ligand antibody
    • Flt 3L antibody
    • Flt3 L antibody
    • FLT3 LG antibody
    • Flt3 ligand antibody
    • Flt3L antibody
    • FLT3L_HUMAN antibody
    • Flt3lg antibody
    • Fms related tyrosine kinase 3 ligand antibody
    • Fms-related tyrosine kinase 3 ligand antibody
    • SL cytokine antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Flt3 ligand/Flt3L with Purified ab52648 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648) at 1/100000 dilution (purified) + Human Flt3 ligand/Flt3L (aa 1 to 235) recombinant protein, 15ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Blocking and diluting buffer: 5% NFDM/TBST.

  • Blocking and diluting buffer: 5% NFDM/TBST.

  • Blocking and diluting buffer: 5% NFDM/TBST.

    See Abreview

  • Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648) at 1/100000 dilution (unpurified) + Recombinant Flt3 ligand/Flt3L at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 26 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical staining of paraffin-embedded human tonsils using unpurified ab52648 at a 1/50 dilution.

References

This product has been referenced in:
  • Traer E  et al. FGF2 from Marrow Microenvironment Promotes Resistance to FLT3 Inhibitors in Acute Myeloid Leukemia. Cancer Res 76:6471-6482 (2016). Read more (PubMed: 27671675) »
  • Taylor RM  et al. VEGF, FLT3 ligand, PlGF and HGF can substitute for M-CSF to induce human osteoclast formation: implications for giant cell tumour pathobiology. Lab Invest 92:1398-406 (2012). Read more (PubMed: 22906984) »
See all 3 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20min
Permeabilization
No
Specification
liver tumor
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 08 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 28 2013

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