Recombinant Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
![Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)](https://www.abcam.com/ps/products/52/ab52648/Images/ab52648-298502-anti-flt3-ligand-flt3l-antibody-ep1140y-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1140Y] to Flt3 ligand/Flt3L
- Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Flt3 ligand/Flt3L antibody [EP1140Y]
See all Flt3 ligand/Flt3L primary antibodies -
Description
Rabbit monoclonal [EP1140Y] to Flt3 ligand/Flt3L -
Host species
Rabbit -
Specificity
The antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand/Flt3L. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.
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Tested applications
Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Human Flt3 ligand/Flt3L aa 150 to the C-terminus.
Database link: P49771 -
Positive control
- IHC: Human liver. WB: recombinant proteinICC/IF: MCF7Flow Cyt: Hela
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Flt3 ligand.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1140Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab52648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/100000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa). | |
| Flow Cyt | 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | 1/180. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2323 Human
- Entrez Gene: 719239 Rhesus monkey
- Omim: 600007 Human
- SwissProt: P49771 Human
- Unigene: 428 Human
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Alternative names
- FL antibody
- Flt 3 ligand antibody
- Flt 3L antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Flt3 ligand/Flt3L with Purified ab52648 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)](https://www.abcam.com/ps/products/52/ab52648/Images/ab52648-299546-anti-flt3-ligand-flt3l-antibody-ep1140y-western-blot.jpg)
Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648) at 1/100000 dilution (purified) + Human Flt3 ligand/Flt3L (aa 1 to 235) recombinant protein, 15ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Flow Cytometry - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)](https://www.abcam.com/ps/products/52/ab52648/Images/ab52648-298517-anti-flt3-ligand-flt3l-antibody-ep1140y-flow-cytometry.jpg)
Flow Cytometry - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Flt3 ligand/Flt3L with ab52648 at 1:20 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) /(Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1:2000 dilution was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)](https://www.abcam.com/ps/products/52/ab52648/Images/ab52648-298373-anti-flt3-ligand-flt3l-antibody-ep1140y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Flt3 ligand with purified ab52648 at 1:180 dilution (2.9 μg/ml). ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)](https://www.abcam.com/ps/products/52/ab52648/Images/ab52648-10-benefits-of-recombinant-antibodies.png)
Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
Datasheets and documents
References (4)
ab52648 has been referenced in 4 publications.
- Yuan X et al. Mesenchymal stem cell therapy induces FLT3L and CD1c+ dendritic cells in systemic lupus erythematosus patients. Nat Commun 10:2498 (2019). PubMed: 31175312
- Traer E et al. FGF2 from Marrow Microenvironment Promotes Resistance to FLT3 Inhibitors in Acute Myeloid Leukemia. Cancer Res 76:6471-6482 (2016). PubMed: 27671675
- Taylor RM et al. VEGF, FLT3 ligand, PlGF and HGF can substitute for M-CSF to induce human osteoclast formation: implications for giant cell tumour pathobiology. Lab Invest 92:1398-406 (2012). PubMed: 22906984
- Tobón GJ et al. The Fms-like tyrosine kinase 3 ligand, a mediator of B cell survival, is also a marker of lymphoma in primary Sjögren's syndrome. Arthritis Rheum 62:3447-56 (2010). IF ; Human . PubMed: 20556820
