RabMAb

Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)

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Overview

  • Product name
    Anti-Flt3 ligand/Flt3L antibody [EP1140Y]
    See all Flt3 ligand/Flt3L primary antibodies
  • Description
    Rabbit monoclonal [EP1140Y] to Flt3 ligand/Flt3L
  • Host species
    Rabbit
  • Specificity

    The antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand/Flt3L. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.

  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Flt3 ligand/Flt3L aa 150-250.

  • Positive control
    • IHC: Human tonsil tissue; human liver. WB: recombinant protein ICC/IF: MCF7 FC: Hela
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as Flt3 ligand.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EP1140Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/100000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/10.
IHC-P 1/180. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100.

Target

  • Function
    Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • FL antibody
      • Flt 3 ligand antibody
      • Flt 3L antibody
      • Flt3 L antibody
      • FLT3 LG antibody
      • Flt3 ligand antibody
      • Flt3L antibody
      • FLT3L_HUMAN antibody
      • Flt3lg antibody
      • Fms related tyrosine kinase 3 ligand antibody
      • Fms-related tyrosine kinase 3 ligand antibody
      • SL cytokine antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
      Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)

      Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Flt3 ligand/Flt3L with Purified ab52648 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
      Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
      Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648) at 1/100000 dilution (purified) + Human Flt3 ligand/Flt3L (aa 1 to 235) recombinant protein, 15ng

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 26 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
      Flow Cytometry - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)

      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)

      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)This image is courtesy of an anonymous Abreview

      Blocking and diluting buffer: 5% NFDM/TBST.

      See Abreview

    • Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
      Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
      Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648) at 1/100000 dilution (unpurified) + Recombinant Flt3 ligand/Flt3L at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 26 kDa
      Observed band size: 22 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)

      Immunohistochemical staining of paraffin-embedded human tonsils using unpurified ab52648 at a 1/50 dilution.

    References

    This product has been referenced in:
    • Traer E  et al. FGF2 from Marrow Microenvironment Promotes Resistance to FLT3 Inhibitors in Acute Myeloid Leukemia. Cancer Res 76:6471-6482 (2016). Read more (PubMed: 27671675) »
    • Taylor RM  et al. VEGF, FLT3 ligand, PlGF and HGF can substitute for M-CSF to induce human osteoclast formation: implications for giant cell tumour pathobiology. Lab Invest 92:1398-406 (2012). Read more (PubMed: 22906984) »
    See all 3 Publications for this product

    Publishing research using ab52648? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Flt3 ligand/Flt3L antibody [EP1140Y]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (liver tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20min
    Permeabilization
    No
    Specification
    liver tumor
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 08 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Flt3 ligand/Flt3L antibody [EP1140Y]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 28 2013

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