Overview

  • Product name

    Fluconazole, Cytochrome P450 inhibitor
  • Description

    Triazole antifungal agent. Cytochrome P450 inhibitor.
  • Biological description

    Triazole antifungal agent. Cytochrome P450 inhibitor (IC50 values are 30.3, 12.3, 13.1 μM for CYP2C9, CYP2C19 and CYP3A4, respectively). Penetrates the blood-brain barrier.
  • Purity

    > 99%
  • CAS Number

    86386-73-4
  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab141065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • HepG2 cells were incubated at 37ºC for 24h with vehicle control (0 µM) and 100 µM of fluconazole (ab141065). Increased expression of cytochrome P450 1A1 (ab3568) correlates with an increase in fluconazole concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3568 at 1/500 dilution and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

References

This product has been referenced in:

  • Niwa T  et al. Effect of antifungal drugs on cytochrome P450 (CYP) 2C9, CYP2C19, and CYP3A4 activities in human liver microsomes. Biol Pharm Bull 28:1805-8 (2005). Read more (PubMed: 16141567) »
  • Yang H  et al. Fluconazole distribution to the brain: a crossover study in freely-moving rats using in vivo microdialysis. Pharm Res 13:1570-5 (1996). Read more (PubMed: 8899853) »

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