Fluo-3 AM, Fluorescent calcium Indicator (ab145254)

Overview

  • Product name

    Fluo-3 AM, Fluorescent calcium Indicator
  • Description

    Cell-permeable fluorescent calcium Indicator.
  • Alternative names

    • 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester
    • Fluo-3 acetoxymethyl ester
  • Biological description

    Cell-permeable acetoxymethyl (AM) ester of the fluorescent calcium indicator Fluo-3. Commonly used in cell-based experiments to detect changes in intracellular calcium levels. Practically nonfluorescent until it is hydrolyzed intracellularly to Fluo-3 and in the presence of Ca2+.

  • Purity

    > 95%
  • CAS Number

    121714-22-5
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    506nm
  • Emission

    526nm
  • Molecular weight

    1129.85
  • Molecular formula

    C51H50Cl2N2O23
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    For more information on AM esters please visit our AM esters FAQ page.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Meng G  et al. Temperature-induced labelling of Fluo-3 AM selectively yields brighter nucleus in adherent cells. Biochem Biophys Res Commun 443:888-93 (2014). Read more (PubMed: 24380862) »
  • Thompson K  et al. Predicting and avoiding subcellular compartmentalization artifacts arising from acetoxymethyl ester calcium imaging probes. The case of fluo-3 AM and a general account of the phenomenon including a problem avoidance chart. Biotech Histochem 87:468-83 (2012). Read more (PubMed: 22984940) »
  • Patel H  et al. Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry. Br J Pharmacol 138:671-7 (2003). Read more (PubMed: 12598421) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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