Fluo-4 AM, Fluorescent labeling reagent (ab241082)

Overview

  • Product name

    Fluo-4 AM, Fluorescent labeling reagent
  • Description

    Fluorescent labeling reagent for the detection of intracellular calcium
  • Biological description

    A fluorescent labeling reagent for the detection of intracellular calcium. Binds to intracellular calcium (Kd = 345 nM).  Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. 


    Fluo-4 AM has spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm.

  • Purity

    > 98%
  • CAS Number

    273221-67-3
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    494nm
  • Emission

    516nm
  • Chemical name

    4-(6-Acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester 

  • Molecular weight

    1096.95
  • Molecular formula

    C51H50F2N2O23
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Store In the Dark. Store under desiccating conditions.
  • Solubility overview

    Soluble in DMSO to 1 mM.

  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c5ccc(C)cc5OCCOc1cc(ccc1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=2c4cc(F)c(cc4OC3=CC(=O)C(F)=CC=23)OCOC(C)=O
  • Source

    Synthetic

Applications

Our Abpromise guarantee covers the use of ab241082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Fluorescent Cell Imaging Use at an assay dependent concentration.

References

This product has been referenced in:

  • Oguro A  et al. 14,15-epoxyeicosatrienoic acid produced by cytochrome P450s enhances neurite outgrowth of PC12 and rat hippocampal neuronal cells. Pharmacol Res Perspect 6:e00428 (2018). Read more (PubMed: 30237892) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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