Product nameFluo-4 AM, Fluorescent labeling reagent
DescriptionFluorescent labeling reagent for the detection of intracellular calcium
A fluorescent labeling reagent for the detection of intracellular calcium. Binds to intracellular calcium (Kd = 345 nM). Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.
Fluo-4 AM has spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm.
4-(6-Acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester
Storage instructionsShipped at 4°C. Store at -20°C. Store In the Dark. Store under desiccating conditions.
Soluble in DMSO to 1 mM.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab241082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Fluorescent Cell Imaging||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241082 has not yet been referenced specifically in any publications.